Rgma (BC023870) Mouse Untagged Clone

CAT#: MC218652

Rgma (untagged) - Mouse RGM domain family, member A (cDNA clone MGC:38550 IMAGE:5353958), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rgma
• Synonyms: C230063O06, MGC38550, MGC69915
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC023870
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTATGGGGAGAGGGGCAGGACGTTCAGCCCTGGGATTGTGGCCGACCCTCGCCTTCCTTCTCTGCA
  GCTTCCCCGCAGCCATCTCCCCCTGCAAAATCCTCAAGTGCAACTCTGAGTTCTGGAGCGCCACGTCATC
  AGGCAGCCACGCCCCTGCCTCCGACGACGTGCCGGAGTTCTGTGCAGCCCTGCGCACCTACGCCCTGTGC
  ACTCGGCGGACAGCCCGCACCTGCCGGGGCGACCTGGCTTACCACTCGGCTGTCCATGGCATAGAGGACC
  TTATGAGCCAGCACAACTGCTCCAAGGACGGCCCCACCTCACAGCCGCGAGTGCGCACGCTCCCGCCAGC
  TGGGGACAGCCAGGAGCGCTCGGATAGCCCCGAGATCTGCCACTATGAGAAGAGTTTCCACAAGCACTCA
  GCTGCCCCCAACTACACTCACTGCGGCCTCTTTGGGGACCCACACCTCAGGACTTTCACAGACCACTTCC
  AGACATGCAAGGTGCAAGGCGCCTGGCCTCTCATCGACAATAATTACCTGAACGTGCAAGTCACCAATAC
  ACCTGTGCTGCCGGGCTCCGCAGCTACCGCCACCAGCAAGCTCACCATCATCTTCAAGAACTTCCAAGAG
  TGTGTGGACCAGAAAGTCTACCAAGCTGAAATGGACGAACTTCCGTCTGCCTTTGCGGACGGCTCCAAAA
  ATGGGGGAGATAAACACGGGGCCAACAGCCTGAAGATCACAGAGAAGGTGTCGGGCCAGCACGTGGAGAT
  CCAGGCCAAGTACATCGGCACCACCATCGTGGTGCGACAGGTGGGCCGCTACCTGACCTTTGCCGTCCGG
  ATGCCCGAGGAGGTAGTCAACGCCGTGGAGGACCGTGACAGCCAAGGCCTCTACCTCTGCCTGCGGGGCT
  GCCCGCTCAACCAGCAGATCGACTTCCAGGCTTTCCGTGCCAACGCTGAAAGCCCTCGCAGGCCAGCAGC
  CGCCAGTCCCTCTCCCGTGGTCCCCGAGACATTCCCCTATGAGACAGCCGTGGCCAAGTGCAAAGAGAAG
  CTGCCCGTAGAAGACCTGTACTACCAGGCCTGTGTCTTCGACCTCCTCACGACTGGTGATGTGAACTTCA
  CGCTGGCTGCCTACTATGCTTTGGAGGATGGCAAGATGCTCCACTCCAACAAGGACAAGCTGCATCTGTT
  TGAAAGGACTCGGGAGCTGCCAGGAGCCGTGGCCGCCGCCGCCGCTGCCACCACATTCCCCTTGGCCCCC
  CAGATTCTCCTTGGCACCATCCCACTTCTGGTCCTCCTGCCTGTGTTGTGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC023870
• Insert Size: 1314 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023870, AAH23870
• RefSeq Size: 3407 bp
• RefSeq ORF: 1313 bp
• Locus ID: 244058
• Cytogenetics: 7 D1
• Gene Summary: Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.[UniProtKB/Swiss-Prot Function]