Arhgap22 (BC038272) Mouse Untagged Clone

CAT#: MC218633

Arhgap22 (untagged) - Mouse Rho GTPase activating protein 22 (cDNA clone MGC:47316 IMAGE:4237331), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgap22
• Synonyms: MGC47316, RHOGAP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC038272
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCTCCCAGCGGGATATGGAGGACTGGGTGCAGGCCATCCGCCGTGTCATCTGGGCCCCGCTGG
  GCAGAGGGATCTTTGGCCAGCGGCTAGAGGACACAGTGCACCATGAGCGGAAGTTTGGTCCCCGGCTGGC
  ACCTCTGCTGGTGGAGCAGTGTGTGGACTTCATCCGGGAGCGAGGACTCAGTGAAGAGGGCCTCTTCCGG
  ATGCCTGGCCAGGCCAACCTGGTGAGGGACCTGCAGGATTCCTTCGACTGTGGGGAGAAGCCGCTGTTTG
  ACAGGTTTCTGGATGAAGTGCAGGCCCACTCAGATGTCAATAAGATGAGTGTCCAGAACTTGGCAACTGT
  CTTTGGTCCTAACATTCTTCGGCCACAGATAGAGGATCCAGTAACCATCATGGAAGGCACGTCTCTGGTT
  CAGCATCTGATGACTGTCCTCATCCGCAAACATGGACAGCTCTTTGCTGCAACCTCCCTTGAAGAACCTG
  CTTCACCACATGGCACAGTAGAGTGGGGCTCTGAAGAGGTCACCAGGGATCACCGGGGTGAGCCTGGCAG
  CCCTGGCTTGCCCACACATAGGACTTCATCTCTGGATGGGCCAGCTGCAGCAGTGCTATCTAGAACTTCG
  CCGCCGCGCCTTGGCAGCCAAACTGGCCCCGCAGCCACCAGCCCTGGGAAGAAGATGCACACTTTGCCTG
  TCTGGAAGTCTTCTTTTCGGCAGCAGGGATCCCGGTCAGAGAGCCCCAAAGGGGTCAACTCATCCTTAGA
  GGTACCCATCATCTCCTCTGGTGGGAACTGGCTCATAAACGGGCTATCGTCTCTTCGCAGCCACCGTCGG
  GCCTCATCGGGGGACCGCCTTAAAGATACAGGCTCGGCACAGAGACTCTCCACCTATGACAATGTGCCGC
  CATCTAGCCAATTTTCTAGCACTGCCAGCGTGGCCAGCACGTCATGGTCTGTAGCCTCGTCCTCCCGAGA
  GGCCTCTGTCAGCAGCTGCACAGCCTGCCGGGCCAGCAACTCTTCTGCCTGCAGCTCCTTACACACCGAG
  TGGGCTCTCGAGCCCTCTCCTCTCCCCAGCAGCAGTGAGGGCCACCAGTCCCCGGACCTGGGCCACAGCC
  TGGATGAGCCATGTGTGGGCAGTGGCAGCAGTGAGCCCAATGACCCAGGCAGCCCCACACAGGCCCATGT
  TCGCCGCTGCCGGGCTCTCCAGGGCCAGGTGGCCGAGCTCAGGGCAGAGTTGTGTCAGCAGAGGACTGAG
  TACAAGAGGAGCCTGAAAAGCATCGAGGAGGGCAGTGCTGACCTGCGGAAGCAGATGTCTCGGTTGGAAG
  AGGAGCTGGACCAGGAGAGGAAGAAGTATGCCATGCTGGAGATCAAGCTCCGGAACTCGGAGCGAGCCCG
  GGAGGATGCGGAGCGCAGGAACCAGCTGCTGCAGAGGGAGATGGAGGAGTTCTTCTCCACCCTGGGGAGC
  TTGACTACAGGGACCAAGGGGTCCAGAGCCCCGGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC038272
• Insert Size: 1509 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC038272, AAH38272
• RefSeq Size: 2108 bp
• RefSeq ORF: 1508 bp
• Locus ID: 239027
• Cytogenetics: 14 B
• Gene Summary: Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. Acts as a GTPase activator for the RAC1 by converting it to an inactive GDP-bound state. Inhibits RAC1-dependent lamellipodia formation. May also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter.[UniProtKB/Swiss-Prot Function]