Tbc1d17 (BC017607) Mouse Untagged Clone

CAT#: MC218617

Tbc1d17 (untagged) - Mouse TBC1 domain family, member 17 (cDNA clone MGC:27642 IMAGE:4507589), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tbc1d17
• Synonyms: MGC27642
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC017607
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGATCCAGCTACAGGGTGGTGTTTGAAAAGGGAGGCGTGTACCTGCACACCAGCGCCCGGAAAC
  ACCAGGATCCAGACTCGCTCATCGCCGGCGTCATACGAGTGGTGGAAAAGGACAGCGACGTCTTTCTGCA
  CTGGGCTCCGGTGGAGGAGGCTGGAGACCCAACCCAGATTCTCTTCAAGAAGGACCCGAGTCGGGGTGAG
  CCCAGCACCTCAGAGGAGGAACCTACCTTTGACCCAGGCTATGAACCTGACTGGGCTGTCATTAGCACGG
  TGCGGCCACAGCCCCACCTGGCAGAGCCCAGGAAAGGTGCAGAGCCCAGTTCCTCCCGGAGCTCCTGGGC
  CTTCTCCGTGAGCCTGGGGGAGCTCAAGTCCATCCGCAGATCCAAGCCAGGCCTCAGCTGGGCCTACCTG
  GTCCTGGTGACCCAGGCTGGAGGCTCCCTGCCTGCCTTACACTTTCACAGAGGGGGTACCCGAGCCCTGC
  TCCGAGTCCTTAGCCGATACCTGCTGTTGGCCAGCTCTCCACAGGACTCCCGCCTCTACTTGGTCTTCCC
  CCAGGACCCCTCTGCCCTGTCCGACTCCTTCCACCACCTGCAGCTCTTTGACCAGGACAGCTCCAATGTG
  GTGTCTCGTTTCCTTCAGGACCCCTACTCCACCACCTTCAGTAGCTTCTCTCGAGTGACCAACTTCTTCC
  GGGGAGCCCTACAGCCGCACCCTGAGGGCGCCTCCTCCCCTAACCTACCCCCGCTGCCAGATGATGAACC
  AGAGCCTGGCTTTGAGGTCATTTCTTGCGTGGAGCTGGGGCAGCGTCCCACAGTGGAGCGTGCTCCTCCA
  GTCACAGAGGAAGAGTGGAACCGCTACGTGGGCCCCGAGGGTCGCCTGCAGAATGTCCCTGAGTTGAAAA
  ACCGGATCTTCTCAGGCGGTCTCAGCCCTGGCCTACGTCGAGAGGCTTGGAAGTTCCTTCTGGGGTACCT
  TAGCTGGGAGAGCTCAGCTGAAGAGCACAAGGCGCATGTGCGGAAGAAAACGGATGAGTACTTCCGAATG
  AAGCTGCAGTGGAAGTCTGTGAGTGCTGAGCAGGAGCGGAGGAACTCACTGCTGCATGGATACCGAAGCC
  TCATTGAGAGAGATGTAAGCCGCACGGACAGGACTAACAAGTTCTATGAAGGCCCTGAGAACCCTGGGCT
  GAGCCTGCTCCATGATATCCTTCTTACCTACTGCATGTACCACTTCGACTTGGGCTACGTTCAGGGTATG
  AGTGACCTCCTGTCCCCGATCCTCTTCGTTGTTCAGAACGAAGTAGATGCCTTCTGGTGTTTCTGCGGCT
  TCATGGAACTTGTGCATGGGAACTTTGAAGAGAGTCAGGAGACTATGAAGCGGCAGCTTGGGCAGCTCCT
  GCTGCTTCTGCGGGTGCTGGATCAGCCACTCTGTGATTTCCTGGATTCTCAGGACTCGGGCTCGCTGTGC
  TTCTGTTTCCGGTGGCTGCTCATCTGGTTCAAGAGGGAATTCCCCTTTCCTGATGTCCTCCGGCTGTGGG
  AGGTGGGCGGCCAGTTGGGGTGCTGTGGACAGGGCTTCCTGGCCCCAATTTGCACCTGCTGGTGGCCTGT
  GCCATCTTGGATATGGAACGGGACACCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC017607
• Insert Size: 1641 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC017607, AAH17607
• RefSeq Size: 2376 bp
• RefSeq ORF: 1640 bp
• Locus ID: 233204
• Cytogenetics: 7 B3
• Gene Summary: Probable GTPase-activating protein for Rab8; its transient association with Rab8 is mediated by OPTN. Inhibits Rab8-mediated endocytic trafficking, such as of transferrin receptor (TfR) and reduces Rab8 recruitnment to tubules emanating from the endocytic recycling compartment (ERC). Involved in regulation of autophagy. Mediates inhibition of autophagy caused by the OPTN variant GLC1E LYS-50; the function requires its catalytic activity, however, the involved Rab is not known (By similarity).[UniProtKB/Swiss-Prot Function]