Trim25 (BC006908) Mouse Untagged Clone

CAT#: MC218581

Trim25 (untagged) - Mouse tripartite motif protein 25 (cDNA clone MGC:6886 IMAGE:2651664), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim25
• Synonyms: AA960166; AL022677; EFP; Zfp147
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC006908
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCTGAATCCTCTGGCCGAGGAGCTGTCCTGCTCCGTCTGCCTGGAGCTCTTCAAGGAACCTG
  TCACCACCCCGTGCGGCCACAACTTCTGCATGTCATGCCTGGATGAGACGTGGGTCGTCCAGGGCCCGCC
  GTACCGGTGCCCGCAGTGTCGCAAAGTGTACCAGGTGCGCCCGCAGCTGCAAAAGAACACGGTAATGTGC
  GCGGTGGTGGAGCAGTTCCTGCAGGCTGAGCAGGCGCGGACCCCGGTGAACGACTGGACGCCCCCTGCCC
  GCTCCTCCGCTTCCAGCGCAGCCACCCAGGTGGCCTGCGACCACTGCCTGACGGAGATCGCAGTGAAGAC
  GTGCCTCGTGTGCATGGCCTCTTTCTGCCAGGAGCACTTGAGGCCTCACTTCGACAGTCCGGCCTTCCAG
  GATCACCCCCTGCAGTCGCCCATCCGTGACCTTCTGCGCCGCAAATGTACCCAGCACAACCGCCTTCGGG
  AATTGTTTTGTCCCGAGCATGGCGAGTGCATCTGCCATATCTGCTTGGTAGAGCACAAGACCTGCTCCCC
  TACGACCCTAAGTCAAGCCAGCGCCGACCTGGAGTACAAACTGAGGAATAAACTCACTATCATGCACAGC
  CACATCAATGGGGCAACCAAAGCATTGGAGGATGTGAGATCCAAGCAACAGTGTGTGCAGGATTCTATGA
  AGAGGAAGATGGAACAGCTGAGACAGGAGTATATGGAAATGAAGGCTGTCATCGACGCAGCAGAGACCAG
  CTCCTTGCGCAGGTTGAAGGAGGAGGAGAAGAGAGTCTACGGAAAATTCGACACCATCTACCAGGTTCTC
  GTCAAGAAGAAGAGTGAGATGCAGAAACTGAAGGCAGAGGTTGAGCTCATTATGGACAAAGGGGATGAGT
  TTGAGTTTCTGGAGAAAGCTGCAAAATTGCAAGGAGAGTCAACAAAACCAGTCTACATCCCTAAGATAGA
  TCTGGATCATGATTTGATAATGGGAATCTACCAAGGGGCGGCGGACCTCAAGAGTGAACTGAAGCACTCC
  ATTAAGAAGCTACAGAAGAAGTCTGAGGAGCACAATGGCTCAGGTAACAAGGGAGACCAGACACAGTCCA
  CTTTTAAACCCGTACAACCTTCGAAGAAGACCATTCCTGTTGCTCCCGGCTCTCCCTCCCACTTCTCACC
  TAACAAGTTGCCCACCTTTGGAGCTCCTGGCCAATCACTGGATTCAAAAGCAACTTCCCCTGATGCCGCA
  CCCAAAGCCAGTGCTGCACAGCCAGACTCTGTAGGAGTCAAGGCTAAGGTGCTAGAAAACTTCTTAACCA
  AGTCCAGAACGGAGCTTCTGGAGTATTTCGTGAAAGTCATCTTCGACTACAATACCGCCCACAACAAAGT
  GTCTCTGTCAAACAAGTACACCACCGCCTCTGTGTCTGATGGGCTCCAGCACTACCGGTCCCATCCCCAG
  AGGTTCACCTACTGCTCTCAGGTCCTGGGGCTGCACTGCTACAAGAACGGCATTCACTACTGGGAGGTGG
  AACTACAGAAGAACAACTTCTGCGGCGTAGGCATCTGCTACGGCAGCATGGAACGGCAGGGCCCCGAGAG
  CCGGCTGGGCCGCAACCCCAACTCATGGTGTGTGGAGTGGTTCAATAACAAGATCTCCGCCTGGCACAAC
  AACGTGGAGAAGACTCTGCCCTCCACCAAGGCCACTCGAGTCGGCGTGCTGCTCAACTGTGACCACGGCT
  TCGTCATTTTCTTTGCGGTCACCGAAAAGGTGCACCTGATGTACAAGTTCAAGGTGGACTTCACCGAAGC
  TCTGTACCCAGCCTTCTGGGTGTTCTCGGCCGGTACCACCCTCTCTATCTGCTCCAAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC006908
• Insert Size: 1881 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC006908, AAH06908
• RefSeq Size: 2602 bp
• RefSeq ORF: 1880 bp
• Locus ID: 217069
• Cytogenetics: 11 C
• Gene Summary: Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58 and IFIH1. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Mediates 'Lys-63'-linked polyubiquitination of IFIH1. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3.[UniProtKB/Swiss-Prot Function]