Cep290 (BC004690) Mouse Untagged Clone

CAT#: MC218579

Cep290 (untagged) - Mouse centrosomal protein 290 (cDNA clone MGC:7859 IMAGE:3501291), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cep290
• Synonyms: MGC7859
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC004690 edited
  CCCACGCGTCCGAGCAAAGAGAAATTGTTAAGAAGCATGAGGAAGACCTTCATGTTCTTCATCACAAATT
  AGAACAACAGGCCGATAATTCACTCAATAAATTCAGACAGACAGCTCAGGATTTACTTAAGCAGTCTCCT
  GCTCCAGTTCCCACCAACAAACATTTCATTCGTCTGGCCGAGATGGAGCAGACAGTAGCAGAACAAGATG
  ACTCTCTGTCCTCACTTTTGACCAAACTAAAGAAAGTATCAAAAGATTTGGAAAAACAAAAAGAAATCAC
  TGAGTTAAAAGTCAGAGAGTTTGAAAATACCAAACTACGGCTCCAAGAAACTCATGCCAGTGAGGTAAAG
  AAAGTGAAAGCAGAGGTAGAGGACTTAAGGCATGCTCTAGCCCAAGCACACAAGGACTCCCAGAGTTTAA
  AGTCTGAACTCCAGGCTCAGAAAGAAGCAAACTCCAGAGCTCCAACAACCACAATGAGGAATCTTGTAGA
  AAGGCTAAAGAGCCAACTAGCCTTGAAAGAGAAGCAACAAAAGGCACTTAGTCGAGCCCTGTTGGAACTT
  CGGTCGGAAATGACAGCAGCAGCTGAGGAACGTATAATCGCTGTAACTTCTCAAAAAGAGGCAAATCTCA
  ATGTTCAACAAGTTGTTGAGCGCCATACTAGAGAGCTAAAGTCACAAATTGAAGATTTAAATGAAAATCT
  TTTAAAATTGAAAGAAGCTCTTAAAACAAGTAAGAACAAAGAAAATTCACTAGCTGATGATTTAAATGAA
  TTAAATAATGAACTGCAAAAAAAGCAAAAAGCTTATAATAAAATCCTTAGAGAGAAAGATGGAATTGATC
  AAGAAAATGATGAACTGAGAAGACAGATTAAAAGACTGTCCAGTGGACTGCAGAGCAAAACTTTGATAGA
  TAACAAGCAAAGTTTAATCGATGAACTTCAAAAGAAAGTTAAAAAACTTGAAAGCCAACTGGAAAGAAAG
  GTGGATGACGTAGACATAAAGCCGGTGAAGGAAAAGAGTAGTAAAGAAGAATTAATTAGGTGGGAAGAAG
  GTAAGAAATGGCAAACCAAAGTAGAGGGACTACGAAACAGACTAAAGGAGAAGGAAGGAGAAGCCCACGG
  CCTGGCAAAGCAGCTGAATACCTTAAAGGAACTTTTTGCCAAAGCTGATAAAGAGAAACTTACTTTGCAG
  AAGAAACTGAAAACAACAGGAATGACTGTTGACCAGGTTTTAGGAGTGCGAGCTTTGGAATCTGAAAAAG
  AGTTGGAAGAGCTAAAAAAGAAAAATCTGGACCTAGAAAATGACATATTATACATGAGGACCCAGCAGGC
  TCTTCCACGAGATTCTGTTGTGGAAGACTTACATTTACAAAATAAATACCTTCAAGAAAAACTTCATACT
  TTAGAAAAAAAACTTTCAAAGGAGAAAATTGTTGCCGAAAATGAACGGCTTCGGAAAGAACTTAAGAAAG
  AAATAGAAGCCTCTGAGAAACTGCGGATAGCTAAGAACAACTTAGAGCTGGTGAACGACAAGATGGCAGC
  TCAACTCGAAGAAACTGGGAAGAGACTACAGTTTGCAGAAAGTAGAGCCCCACAGCTGGAAGGTGCTGAC
  AGCAAGAGCTGGAAGTCAATTGTGGTCTCAAGAGTGTATGAGACCAAGATGAAAGAGCTTGAAAGTGACA
  TTGCCAAAAAGAATCAAAGTATCACTGACCTTAAACAGCTTGTAAGAGAAGCAACAGAGAGAGAACAGAA
  AGCTAAGAAATACACTGAAGACCTTGAACAACAGATTGAGATCCTCAAAAATGTTCCTGAAGGTGCCGAG
  ACAGAGCAAGAGCTTATACGGGAACTCCAGCTTCTTAGATTAGCCAATAATCAGCTGGATAAAGAAAGGG
  CAGAATTAATCCATCAGATAGAAATTAACAAGGACCAAACCAGAGCTGACAGTAGCATACCTGATTCTGA
  TCAACTAAAGGAAAAGATAAATGACCTGGAGACACAACTCAGAAAGTTGGAGCTAGAAAAGCAACATTCG
  AAGGAGGAAGTTAAAAAGCTGAAAAAAGAACTGGAAAATTTTGATCCTTCATTTTTTTGAAGAAATTGAA
  GACCTGAAGTATAATTATAAGGAAGAAGTGAAAAAGAATATCCTATTAGAAGAGAAGCTAAAAAAACTGT
  CGGAACAGTTTGGATTTGAACTGCCTAGTCCTCTTGCTGCTTCTGAACACTCGGAAGATGGAGAAAGTCC
  TCATAGTTTCCCTATTTATTAGTGCCCTCGTGTAACTGCAGGTTTAAAACTATTTTATGAGTGAAAAAAG
  TTTCAGCTGGAAATCAAGCAGTCTCTGTGTTATAGAATTTACAGCTCAAAATATTGTATTTTTTTTGTTT
  AAAAAAATCATATTTAGTTTTAACTTGTTAGAAAAGTATATTTTTGAAGACTTCATTAATTTATGACTAT
  ACAGTGCACAATGAGCTATGATAAATATTACTATTTTCATAATAACTTAAATTTTCAAAAAGCATAAAGG
  TAAGCTTATATCAATTTACTATTTAAAATTGATTATTTTATATTTATTTTTGTGTTAAGATAGTCAAATC
  CTAACGATGATTTCTATAAATACCTAGAAACATGTTTAGAAGTTGACAGGTTTTGCCAAACAGCTAGCAA
  AATTAAATGCAAACTATTCAGTCTTAGAAAATCACAATAATCTCAACAATTAGTTCCATTTTATACATCA
  AGTATTATGTCTTTGAGTCTCTCAAATTTCTGTTTATCTATTTTTGAAAACTGATTAAACAAAGACTGGT
  
• Restriction Sites: SgfI-MluI
• ACCN: BC004690
• Insert Size: 1176 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC004690.1
• RefSeq Size: 2831 bp
• RefSeq ORF: 1907 bp
• Locus ID: 216274
• Cytogenetics: 10 51.48 cM
• Gene Summary: Involved in early and late steps in cilia formation (PubMed:21565611). Its association with CCP110 is required for inhibition of primary cilia formation by CCP110 (By similarity). May play a role in early ciliogenesis in the disappearance of centriolar satellites and in the transition of primary ciliar vesicles (PCVs) to capped ciliary vesicles (CCVs). Required for the centrosomal recruitment of RAB8A and for the targeting of centriole satellite proteins to centrosomes such as of PCM1 (By similarity). Required for the correct localization of ciliary and phototransduction proteins in retinal photoreceptor cells; may play a role in ciliary transport processes (PubMed:16632484). Required for efficient recruitment of RAB8A to primary cilium (By similarity). In the ciliary transition zone is part of the tectonic-like complex (also named B9 complex) which is required for tissue-specific ciliogenesis and may regulate ciliary membrane composition (PubMed:21725307). Involved in regulation of the BBSome complex integrity, specifically for presence of BBS2, BBS5 and BBS8/TTC8 in the complex, and in ciliary targeting of selected BBSome cargos. May play a role in controlling entry of the BBSome complex to cilia possibly implicating IQCB1/NPHP5 (By similarity). Activates ATF4-mediated transcription (By similarity).[UniProtKB/Swiss-Prot Function]