D14Ertd231e (BC028953) Mouse Untagged Clone

CAT#: MC218558

D14Ertd231e (untagged) - Mouse DNA segment, Chr 14, ERATO Doi 231, expressed (cDNA clone MGC:38896 IMAGE:5362016), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: D14Ertd231e
• Synonyms: BC028953; D14Ertd231e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC028953
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACTGTATTGCCTGTCAGGGCACCCAACTTTACCATGCAACGTACTCAAATTCAAATCCACTACTA
  TTATGTTGGACTGTGGACTGGACATGACTTCCACCCTCAATTTCCTCCCTTTGCCTCTTGTTCAAAGCCC
  CAGGCTATCTAATCTTCCCGGATGGTCTCTAAAAGACGGAAATGCTTTTTTGGACAAGGAGCTAAAAGAG
  TGCTCCGGTCATGTATTTGTGGATTCTGTGCCTGAATTCTGCTTACCAGAGACCGAGCTAATAGACCTGT
  CTACAGTCGATGTGATCCTCATCTCTAACTACCACTGCATGATGGCGCTGCCCTACATCACCGAGCACAC
  AGGATTCACAGGCACAGTGTATGCCACAGAACCCACCATGCAGATCGGCAGGCTTCTCATGGAAGAGCTG
  GTGAATTTCATCGAAAGAGTGCCAAAGGCCCAGTCTGCCTCCTTGTGGAAGAATAAGGATATACAGCGGC
  TGCTGCCTTCTCCCCTCAAGGATGCAGTGGAAGTGTCAACCTGGAGAAGATGCTACACGATGCAGGAGGT
  GAACTCGGCCCTCAGTAAAATCCAGCTGGTGGGATATTCCCAGAAAATCGAGCTGTTTGGTGCTGTCCAG
  GTGACCCCTCTGAGCTCTGGCTATGCCCTTGGAAGCTCCAACTGGATTATCCAGTCACATTATGAGAAAG
  TATCTTACGTCTCTGGATCCTCCTTGCTTACCACACACCCTCAGCCCATGGACCAAGCTTCTCTCAAAAA
  CAGTGACGTTCTGATTCTCACAGGGCTGACACAGATCCCTACTGCAAACCCGGACGGCATGGTGGGCGAG
  TTCTGCAGCAACCTCGCTCTGACAGTTCGGAATGGAGGAAACGTGTTGGTCCCTTGCTATCCTTCAGGCG
  TGATCTATGACCTCCTGGAGTGCTTATATCAATACATTGACTCCGCTGGCCTCTCCAACATCCCTTTCTA
  CTTCATCTCCCCCGTTGCCAACAGTTCACTCGAGTTTTCCCAGATTTTTGCTGAGTGGCTTTGTCACAAC
  AAACAGAGTAAAGTTTACCTCCCAGAACCACCTTTCCCACATGCAGAGCTCATTCAGACGAATAAGCTGA
  AGCACTACCGCAGCATCCACGGTGACTTCAGCAATGACTTCAGACAGCCGTGTGTGCTGTTCACCGGACA
  TCCTTCCCTCCGCTTTGGGGACGTCGTCCACTTCATGGAGCTCTGGGGGAAGTCCAGCCTCAACACCATC
  ATATTCACAGAACCCGATTTCTCCTACCTGGAGGCCTTGGCTCCTTACCAGCCGCTGGCCATGAAGTGCA
  TCTACTGCCCAATTGACACTCGACTGAATTTCATCCAGGTGTCAAAGCTGCTTAAAGAAGTACAGCCCCT
  GCACGTGGTCTGTCCTGAGCAGTACACCCAGCCACCGCCAGCCCAGGCCCACAGGATGGACCTCATGATC
  GACTGCCAGCCACCAGCCATGTCCTATCGACGGGCTGAGGTCCTTGCCCTGCCCTTCAAACGACGCTATG
  AGAAAATTGAAATCATGCCAGAGCTGGCAGACTCCCTGGTGCCCATGGAAATCAAGCCTGGCATCTCCTT
  GGCGACTGTCTCAGCTGTGCTGCATACGAAGGATAACAAACATGTGCTTCAGCCCCCTCCCAAGCCCACC
  CAGCCCACCAGCAGTAAGAAGAGGAAGCGGGTGAACGAGGACATTCCTGACTGCAAAGTACTGAAGCCTC
  TGCTGAGTGGCTCCATCCCCGTAGAGCAGTTTGTGCAGACCCTGGAGAAGCATGGCTTCAGTGACATTAA
  GGTGGAAGACACAGCCAAGGGACATATTGTCCTGCTGCAGGAAGCCGAGACACTCATCCAGATTGAAGAA
  GACTCCACTCACATCATCTGTGACAATGACGAGACACTGAGGGTCCGACTGCGGGACCTTGTCCTCAGGT
  TCCTGCAGAAGTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC028953
• Insert Size: 1977 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC028953, AAH28953
• RefSeq Size: 2212 bp
• RefSeq ORF: 1976 bp
• Locus ID: 210925
• Cytogenetics: 14 33.81 cM
• Gene Summary: Component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex.[UniProtKB/Swiss-Prot Function]