Tas1r1 (BC070448) Mouse Untagged Clone

CAT#: MC218536

Tas1r1 (untagged) - Mouse taste receptor, type 1, member 1 (cDNA clone MGC:99408 IMAGE:30619268), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tas1r1
• Synonyms: TR1, T1r1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC070448
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTTTCTGGGCAGCTCACCTGCTGCTCAGCCTGCAGCTGGCCGTTGCTTACTGCTGGGCTTTCAGCT
  GCCAAAGGACAGAATCCTCTCCAGGTTTCAGCCTCCCTGGGGACTTCCTCCTGGCAGGCCTGTTCTCCCT
  CCATGCTGACTGTCTGCAGGTGAGACACAGACCTCTGGTGACAAGTTGTGACAGGTCTGACAGCTTCAAC
  GGCCATGGCTATCACCTCTTCCAAGCCATGCGGTTCACCGTTGAGGAGATAAACAACTCCACAGCTCTGC
  TTCCCAACATCACCCTGGGGTATGAACTGTATGACGTGTGCTCAGAGTCTTCCAATGTCTATGCCACCCT
  GAGGGTGCTCGCCCAGCAAGGGACAGGCCACCTAGAGATGCAGAGAGATCTTCGCAACCACTCCTCCAAG
  GTGGTGGCACTCATTGGGCCTGATAACACTGACCACGCTGTCACCACTGCTGCCCTGCTGAGCCCTTTTC
  TGATGCCCCTGGTCAGCTATGAGGCGAGCAGCGTGATCCTCAGTGGGAAGCGCAAGTTCCCGTCCTTCTT
  GCGCACCATCCCCAGCGATAAGTACCAGGTGGAAGTCATAGTGCGGCTGCTGCAGAGCTTCGGCTGGGTC
  TGGATCTCGCTCGTTGGCAGCTATGGTGACTACGGGCAGCTGGGCGTACAGGCGCTGGAGGAGCTGGCCA
  CTCCACGGGGCATCTGCGTCGCCTTCAAGGACGTGGTGCCTCTCTCCGCCCAGGCGGGTGACCCAAGGAT
  GCAGCGCATGATGCTGCGTCTGGCTCGAGCCAGGACCACCGTGGTCGTGGTCTTCTCTAACCGGCACCTG
  GCTGGAGTGTTCTTCAGGTCTGTGGTGCTGGCCAACCTGACTGGCAAAGTGTGGATCGCCTCCGAAGACT
  GGGCCATCTCCACGTACATCACCAATGTGCCCGGGATCCAGGGCATTGGGACGGTGCTGGGGGTGGCCAT
  CCAGCAGAGACAAGTCCCTGGCCTGAAGGAGTTTGAAGAGTCCTATGTCCAGGCAGTGATGGGTGCTCCC
  AGAACTTGCCCAGAGGGGTCCTGGTGCGGCACTAACCAGCTGTGCAGGGAGTGTCACGCTTTCACGACAT
  GGAACATGCCCGAGCTTGGAGCCTTCTCCATGAGCGCTGCCTACAATGTGTATGAGGCTGTGTATGCTGT
  GGCCCACGGCCTCCACCAGCTCCTGGGATGTACCTCTGGGACCTGTGCCAGAGGCCCAGTCTACCCCTGG
  CAGCTTCTTCAGCAGATCTACAAGGTGAATTTCCTTCTACATAAGAAGACTGTAGCATTCGATGACAAGG
  GGGACCCTCTAGGTTATTATGACATCATCGCCTGGGACTGGAATGGACCTGAATGGACCTTTGAGGTCAT
  TGGTTCTGCCTCACTGTCTCCAGTTCATCTAGACATAAATAAGACAAAAATCCAGTGGCACGGGAAGAAC
  AATCAGGTAATGGGACAAGGTCGCTCACCAGGTGACTGCTTTGTTACCAGAATCTGGGGAGTGATGCGAA
  CCCAGAGCAGGATGGGGCCCCACACATCAGGCTACCTCAGGTCTGCAGTCCTGCCAGGGATGCCAGCTGC
  AGTGTTGTCAAACAGATGTCGGAGAGGGGACCTGGAGGGCAGTGGCACAGATCCCTCTTCTCAGTCTCCC
  CTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC070448
• Insert Size: 1068 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC070448
• RefSeq Size: 4723 bp
• RefSeq ORF: 1685 bp
• Locus ID: 110326
• Cytogenetics: 4 82.83 cM
• Gene Summary: Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate) and also to most of the 20 standard L-amino acids, but not to their D-enantiomers or other compounds. Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses.[UniProtKB/Swiss-Prot Function]