Zbtb48 (BC005515) Mouse Untagged Clone

CAT#: MC218517

Zbtb48 (untagged) - Mouse zinc finger and BTB domain containing 48 (cDNA clone MGC:7588 IMAGE:3493611), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zbtb48
• Synonyms: 0610011D15Rik; AI327031; Hkr3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC005515
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGGTTCCTTCGTGCAGCACAGCGTGAGGGTTCTGCAGGAACTCAACAAGCAGCGCGAGAAGGGCC
  AGTATTGCGACGCCACGCTGGACGTGGGGGGCCTGGTGTTCAAGGCGCACTGGAGTGTCCTGGCGTGTTG
  CAGCCATTTCTTCCAGAGAATCTACGGTGATGGCACAGGGGGCAGCGTTGTCCTCCCAGCGGGCTTTGCT
  GAGATTTTTGGCCTCCTGCTGGACTTTTTCTACACCGGCCACCTCGCCCTCACCTCAGGGAACAGGGATC
  AGGTACTCTTGGCAGCCAAAGAGTTGCGGGTGCCTGAGGCTGTGGAGCTATGTCAGAGCTTCCAGCCCCA
  AACCTCAGTGGGACAGGCACAGAGTGGACTGGGTCAACCTGCCTCCCAGGACGTGAAAAGCCACCTGAAG
  GAGCCGACAGACTTGGATGAGGAGGAAGTTTTCAAGACTCTGAGCCTGGCCTCCGTGGATCAGGAGCCCA
  GAGACACTGAGCAGCCCCAGCTGGGTACACCTGCTCAGAGCAGCACGGCCTTCCTCTGTGGGAAACTCAC
  GCAGGCTTTGAAGCCTTCTCCTTCAGAGGACAAAGAGTCTGAGGACTGCAAAGAGCCCCCAAGGCCCTTC
  GAGGCTGGAGGTGCCCCGCTGCAGGGCGAGAGCAATGAGTGGGAAGTGGTGGTCCAAGTGGAGGACGACA
  GGGACGGTGATTACGTATCTGAGCCTGAGACTGTGCTGACCAGAAGGAAGTCAAAAGTGATTAGAAAACC
  CTGTGCAGCCGAGCCAGCCCTGGGTGCGGGTTCTCTCACAGCCGAGCCCACTGACAGCAGAAAAGGTGCA
  GCCGTTCCGGTCGAATGTCCCACATGTCACAAAAAGTTCCTCAGCAAATATTACCTGAAAGTCCACAACA
  GGAAACACACTGGGGAGAAACCCTTCGAGTGTCCCAAATGTGGGAAGTGTTACTTCCGCAAGGAGAATCT
  CTTGGAACATGAAGCCAGGAATTGCATGAACCGCTCAGAACAGGTCTTCACATGCTCCGTATGCCAGGAG
  ACCTTCCGACGGAGGATGGAGCTGCGCTTGCACATGGTGTCTCACACAGGGGAGATGCCCTACAAGTGTT
  CCTCCTGCTCACAGCAGTTCATGCAGAAGAAAGACTTGCAGAGCCACATGATCAAGCTGCACGGAGCCCC
  TAAACCCCACGCAGTAAGTGCCAGTCAGGGCTGGGGTTCCGGTGGCTCTGGGACAGCTCAATCCTGTCCT
  CACCTGGATGTGCTCCCTCTGCCTGTCTCACCCACCTGCAGTGCCCCACTTGTGCCAAGTGCTTCCTGTC
  TAGGACGGAGCTACAGCTGCACGAGGCTTTTAAGCATCGTGGAGAAAAGCTCTTTGTGTGTGAGGAATGC
  GGGCACCGGGCCTCGAGCCGCAACGGGCTGCAGATGCACATCAAGGCCAAGCACAGGAATGAAAGGCCTT
  ATGTCTGTGAGTTCTGCAGCCATGCCTTCACCCAGAAGGCCAACCTCAACATGCACCTGCGCACGCACAC
  CGGCGAGAAGCCTTTCCAGTGCCACCTCTGTGGGAAGACCTTCCGCACCCAAGTTTGGACAAGCACAACC
  GCACCCACACGGGCGAGAGGCCTTTCAGCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC005515
• Insert Size: 1644 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC005515, AAH05515
• RefSeq Size: 2418 bp
• RefSeq ORF: 1643 bp
• Locus ID: 100090
• Cytogenetics: 4 E2
• Gene Summary: Telomere-binding protein that acts as a regulator of telomere length. Directly binds the telomeric double-stranded 5'-TTAGGG-3' repeat. Preferentially binds to telomeres that have a low concentration of shelterin complex and acts as a regulator of telomere length by initiating telomere trimming, a process that prevents the accumulation of aberrantly long telomeres. Also acts as a transcription regulator that binds to promoter regions. Regulates expression of a small subset of genes, including MTFP1. Regulates expression the J and/or S elements in MHC II promoter. Acts as a negative regulator of cell proliferation by specifically activating expression of ARF, a tumor suppressor isoform of CDKN2A.[UniProtKB/Swiss-Prot Function]