Kars (BC027356) Mouse Untagged Clone

CAT#: MC218510

Kars (untagged) - Mouse lysyl-tRNA synthetase (cDNA clone MGC:37041 IMAGE:4950333), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kars
• Synonyms: AA589550; AL024334; AL033315; AL033367; D8Ertd698e; D8Wsu108e; LysRS; mKIAA0070
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC027356
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGATGCAAGCTGCTGTCAGGCTTGTTAGGGGGGCCCTGCGCCAAACCTCATGGGCAGAATGGGGTC
  AGAGGGAACTTCGACTGGGCCACCTTGCTCCTTTCACAACGCTCCACAAGGACCAGCCACTTTCTGATAG
  AAGAAGTGAGCTGAAGAGGCGTCTGAAAGCTGAGAAGAAACTGGCAGAGAAGGAGGCCAAGCAGAAAGAG
  CTGAGTGAGAAACAGCTAAACCAGACTGCTTCCGCTCCCAACCACACGGCTGACAATGGCGTGGGTGCTG
  AGGAGGAGACTCTGGACCCAAATCAATACTACAAGATCCGAAGTCAAGCCGTCCAGCAGCTGAAGGTCAC
  TGGGGAGGATCCGTACCCACACAAGTTCCACGTGGACATCTCACTCACTCAGTTCATCCAAGAATATAGT
  CACCTGCAGCCTGGGGACCACCTGACTGATGTCACCCTCAAAGTGGCAGGCCGCATCCACGCCAAGAGGG
  CCTCTGGAGGGAAGCTCATCTTCTATGACCTGCGAGGAGAGGGGGTCAAGTTACAAGTCATGGCCAACTC
  CAGGAATTACAAATCAGAGGAGGAATTTGTTCATATCAATAACAAACTGCGCCGGGGAGACATAATTGGA
  GTTGAGGGCAATCCCGGGAAAACCAAGAAGGGCGAGCTGAGCATCATCCCCCAGGAGATCACGCTGCTGT
  CCCCCTGCTTGCACATGCTGCCTCATCTTCACTTTGGCCTCAAAGACAAGGAAACACGGTATCGTCAAAG
  ATACTTGGACTTGATCCTGAACGACTTTGTGAGGCAGAAATTTATCGTCCGCTCTAAGATCATCACATAC
  ATAAGAAGTTTCTTGGATGAGCTGGGCTTCCTAGAGATCGAAACTCCCATGATGAACATCATTCCCGGGG
  GAGCTGTGGCCAAGCCTTTCATCACCTATCACAATGAGCTGGACATGAACTTGTATATGAGAATTGCTCC
  AGAACTCTACCACAAGATGCTGGTGGTTGGTGGCATTGACCGGGTTTATGAAATTGGGCGCCAGTTCCGG
  AACGAAGGGATTGATTTGACTCACAATCCTGAGTTCACCACCTGTGAGTTCTACATGGCCTATGCAGACT
  ATCATGACCTCATGGAGATCACAGAGAAGATGCTGTCAGGGATGGTGAAGAGCATTACAGGCAGTTACAA
  GATCACCTACCACCCAGATGGGCCGGAAGGCCAAGCCTACGAGGTCGACTTCACCCCACCCTTCCGAAGA
  ATCAGCATGGTAGAAGAGCTTGAGAAAGCCCTAGGTGTGAAGCTGCCAGAAACCAGTCTCTTTGAAACTG
  AAGAAACTCGGAAAATTCTTGATGATATTTGTGTTGCAAAAGCTGTTGAATGCCCCCCACCTCGGACCAC
  AGCCAGGCTCCTTGATAAGCTCGTTGGCGAGTTCCTCGAAGTCACATGCATCAGCCCTACCTTCATCTGT
  GATCACCCACAGATCATGAGTCCTTTGGCCAAATGGCACCGCTCCAAAGAGGGTCTCACGGAGCGCTTTG
  AGCTGTTTGTCATGAAGAAGGAGATATGCAATGCCTATACTGAGCTGAATGACCCCGTGCGGCAGAGGCA
  GCTGTTTGAGGAGCAGGCCAAGGCCAAGGCTGCCGGTGATGACGAGGCCATGTTCATAGACGAGAACTTC
  TGTACTGCCCTGGAATACGGGCTGCCTCCCACAGCGGGCTGGGGCATGGGCATTGATCGGCTCACCATGT
  TTCTCACCGATTCCAACAATATCAAGGAAGTACTTCTGTTTCCTGCCATGAAGCCAGAGGACAAGAAGGA
  AACTGCAGCGACCACTGAAACCCCAGAGAGCACAGAGGCCAGCCCCTCTGTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC027356
• Insert Size: 1875 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027356, AAH27356
• RefSeq Size: 2205 bp
• RefSeq ORF: 1874 bp
• Locus ID: 85305
• Cytogenetics: 8 58.27 cM
• Gene Summary: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.[UniProtKB/Swiss-Prot Function]