Smarcd1 (BC026783) Mouse Untagged Clone

CAT#: MC218507

Smarcd1 (untagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1 (cDNA clone, (10ug)


  "BC026783" in other vectors (4)

Reconstitution Protocol

USD 503.00

3 Weeks*

Size
    • 10 ug

Product Images

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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Smarcd1
Synonyms AA407987; Baf60a; D15Kz1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC026783
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCCGGCGCCGGGTCAAGGGCTGTACCGCTCTCCGATGCCCGGGGCGGCCTATCCGAGACCAGGTA
TGCTGCCAGGTAGCCGAATGACACCTCAGGGACCTTCCATGGGACCTCCTGGCTATGGGGGGAACCCTTC
AGTCCGACCTGGTCTGGCCCAGTCAGGGATGGACCAGTCCCGCAAGAGACCTGCACCTCAACAGATCCAG
CAGGTCCAGCAGCAGGCGGTCCAAAATCGAAATCACAATGCAAAGAAAAAGAAGATGGCTGACAAAATCC
TACCTCAAAGGATTCGGGAACTGGTCCCAGAATCACAGGCCTACATGGATCTCCTGGCTTTTGAAAGGAA
ACTGGACCAGACTATTATGAGGAAGCGGCTAGATATCCAGGAGGCCTTGAAACGTCCCATCAAGCAAAAA
CGGAAGCTGCGAATTTTCATTTCTAACACGTTCAATCCGGCTAAGTCGGACGCGGAGGATGGGGAAGGGA
CGGTGGCTTCCTGGGAGCTCCGGGTAGAAGGCCGGCTCCTGGAGGACGCGGCCTTGTCCAAATATGACGC
CACCAAGCAAAAGAGAAAGTTCTCTTCCTTTTTTAAGTCCTTGGTGATCGAACTGGACAAAGACCTCTAT
GGCCCAGACAACCATCTGGTAGAATGGCACAGGACCGCCACTACCCAGGAGACCGATGGCTTCCAGGTGA
AGCGGCCAGGAGATGTGAATGTACGGTGTACTGTCCTGCTGATGCTGGACTACCAGCCCCCCCAGTTTAA
ATTAGACCCTCGCCTGGCTCGGCTCTTGGGCATCCATACCCAGACACGTCCAGTGATCATCCAAGCACTG
TGGCAGTATATTAAAACACACAAGCTCCAGGACCCTCACGAGCGAGAGTTTGTTCTCTGTGACAAGTACC
TCCAGCAGATCTTTGAATCTCAGCGGATGAAGTTCTCAGAGATCCCTCAGCGGCTCCACGCCTTGCTTAT
GCCACCAGAGCCCATCATCATCAATCATGTCATCAGTGTGGACCCAAATGACCAGAAAAAGACCGCGTGC
TATGACATTGACGTGGAGGTGGATGACACTCTGAAGACCCAGATGAACTCTTTCCTGTTGTCCACTGCCA
GCCAGCAGGAGATCGCCACTCTAGACAACAAGATCCATGAGACGATAGAGACCATCAACCAGCTGAAGAC
CCAGCGAGAGTTCATGTTGAGCTTTGCCCGAGACCCTCAGGGTTTCATCAATGATTGGCTTCAGTCCCAG
TGCAGGGACCTCAAGACGATGACTGATGTGGTGGGTAACCCGGAAGAGGAGCGTCGTGCTGAGTTCTACT
TCCAGCCCTGGGCTCAGGAGGCTGTGTGCCGATACTTCTACTCCAAGGTGCAGCAGAGGCGGCAAGAGTT
AGAGCAAGCCCTGGGAATCCGAAACACATAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Restriction Sites SgfI-MluI     
ACCN BC026783
Insert Size 1431 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC026783, AAH26783
RefSeq Size 3086 bp
RefSeq ORF 1430 bp
Locus ID 83797
Cytogenetics 15 56.13 cM
Gene Summary Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (By similarity). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (PubMed:17640523). Has a strong influence on vitamin D-mediated transcriptional activity from an enhancer vitamin D receptor element (VDRE). May be a link between mammalian SWI-SNF-like chromatin remodeling complexes and the vitamin D receptor (VDR) heterodimer. Mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation (By similarity).[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.