Phf23 (BC033533) Mouse Untagged Clone

CAT#: MC218494

Phf23 (untagged) - Mouse PHD finger protein 23 (cDNA clone MGC:25514 IMAGE:2655211), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Phf23
• Synonyms: JUNE-1, RP23-172M21.13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC033533
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCCCAGTCCCGAAGATCCGCCTCCGACTCTTAAACCAGAGACTCAGCCACCGGAGAAAAGGC
  GGAGAACCATTGAGGATTTTAACAAATTCTGCAGTTTTGTCTTGGCCTATGCTGGTTACATCCCCCCTAG
  CAAAGAGGCTCCTGACAGTGCTACTCTGCTTGAGAAGATGAAGCTCAAGGACTCTCTTTTTGATCTGGAT
  GGGCCCAAAGTGGCATCTCCACTCTCTCCCACATCATTGACGCATACCTCCCGGCCCCCCGCTGCTCTCG
  CCCCAGTGCCGCTGTCCCAGGGGGACCTCTCCCAGCCTCGAAAGAAGGACCGAAAGAATAGAAAGTTGGG
  ACCAGGAGGTGGGGCTGGCTTTGGGGTGCTTCGGAGACCTCGACCAGCTCCTGGGGATGGGGAAAAGCGG
  TCTCGAATCAAGAAGAGCAAGAAGCGGAAGTTGAAAAAGGCAGATCGGGGAGATAGACTCCCACCTCCTG
  GCCCTCCTAGGGCTCCTCCCAGTGATACAGACTCTGAAGAGGAGGAGGAAGAGGAAGAAGAGGAAGATGA
  TGAGGAAGAGATGACAGTGGGGGGTGGAGTCCCAGCCCCTGTGCTCCCAACACCCCCTGAGGCCCCCAGG
  CCCCCTGTTACAGTGCACTCTGAAGGTGCTCCCCCTACTGACAGTGAAGGCAAAGATGTGGGCAGCACAG
  AAACAAGCCAAGATGGAGATGCTAGCTCCAGTGAAGGCGAGATGCGGGTCATGGATGAGGACATCATGGT
  AGAATCAGGTGACGACTCCTGGGATCTGATCACGTGTTACTGCAGAAAGCCCTTTGCGGGGCGGCCCATG
  ATTGAGTGCAGCCTCTGTGGGACGTGGATCCACCTCTCCTGTGCTAAGATTAAGAAGACCAACGTTCCCG
  ACTTCTTTTATTGCCAGAAATGCAAGGAACTTCGGCCAGAGGCCCGGCGGTTAGGGGGTCTCCCCAAATC
  TGGAGAACCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC033533
• Insert Size: 993 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC033533, AAH33533
• RefSeq Size: 1728 bp
• RefSeq ORF: 992 bp
• Locus ID: 78246
• Cytogenetics: 11
• Gene Summary: Acts as a negative regulator of autophagy, through promoting ubiquitination and degradation of LRSAM1, an E3 ubiquitin ligase that promotes autophagy in response to starvation or infecting bacteria.[UniProtKB/Swiss-Prot Function]