Mindy1 (NM_199475) Mouse Untagged Clone

CAT#: MC218476

Fam63a (untagged) - Mouse family with sequence similarity 63, member A (Fam63a), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mindy1
• Synonyms: 1810005H09Rik; 4930504E06Rik; AA562754; AA960384; AW561904; cI-40; mKIAA1390; ni
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218476 representing NM_199475
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACAACCTCAGACTGAGAACCCAGCCCCTAGTAAGGCCACTAGTGCAGAAACTGTTGAATCTGAAA
  ACCATGAGGCCCTGTCTGGACCAGAGAAGCATCCTCAGGACAAGGATGGTGCAGATGCTGATGGAGCAGC
  TGGAGAACAGGAGCCAGGAGACCAAACTTTGCCACCAGCCCAGGATGGTGAAAATCTTGAGTGTCCTCCT
  CCTGAAGCTAGCTCAAGTCCTCCTGGGCCAGCTTGTGGGACGTCACCTAAGGTAGAGACAGCAGAGGTGT
  GTTCCAGGCCACAGGAACTTCCCCAGTCCCCCAGGATCCAACAGCCTGAGCTAGATTTCTACTGTGTCAA
  GTGGATCCCCTGGAAGGGAGAACGGACCCCGATCATCACCCAGAGCACCAATGGTCCGTGTCCTCTTCTA
  GCCATCATGAACATCCTTTTTCTTCAGTGGAAGGTGAAGCTGCCCCCTCAGAAGGAAGTGATCACATCAG
  ATGAGCTCCTGACACACCTTGGAAACTGCCTTCTGTCCATCAAACCCCAAGAGAAGTCTGAGGGCCTTCA
  ACTTAACTTTCAGCAGAATGTGGACGATGCAATGACGGTGCTCCCGAAACTGGCCACAGGTCTGGACGTC
  AATGTGCGATTCACGGGCGTCTCCGACTTTGAGTATACGCCGGAATGCAGCATCTTTGACTTGCTGGGAA
  TACCTCTATACCATGGCTGGCTGGTGGATCCACAGAGTCCCGAGGCTGTGAGTGCTGTTGGGAAACTGAG
  TTACAACCAGCTGGTAGAGAAGATCATCACCTGTAAACACTCTAGTGACTCCAACCTCGTGACAGAAGGC
  TTGGTTGCGGAGCAGTTCCTGGAGACCACTGCGGCACAGTTGACCTACCATGGCCTGTGCGAGCTCACAG
  CAGCTGCCACGGAAGATGAACTTAGTGTCTTCTTTCGAAACAACCACTTCAGCACGATGACTAAGCACAA
  GAGTCACCTGTACCTACTGGTCACTGACCAAGGTTTTCTACAGGAGGAGCAGGTGGTATGGGAGAGCTTG
  CATAACGTGGATGGTGACAGCTGCTTTTGTGACTCGGACTTCCACCTAAGTCATTCCCTAGGCAAAAGCC
  ATGGAGCAGAAGGTGGGGGTGGCTCTCCAGAGAAGCAGCTGCAAGTGGACCAGGACTACCTTATTGCCTT
  ATCCCTGCAGCAGCAGCAGCAGCCACAAGGCACGTTGGGCCTCAGTGACCTGGAACTGGCCCAACAACTT
  CAACAAGAAGAGTATCAACAACAGCAAGCAGTTCAACCTGTGCGAACAAGGGCTCCTTCGCCCCAGGGGA
  GAGGAGCCACATCTGGACGTCCAGCTGGAGAGCGGCGGCAGAGGTCAAAGACTGAGTCAGACTGTGTTCT
  GCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_199475
• Insert Size: 1407 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199475.1, NP_955769.1
• RefSeq Size: 2850 bp
• RefSeq ORF: 1407 bp
• Locus ID: 75007
• UniProt ID: Q76LS9
• Cytogenetics: 3 F2.1
• Gene Summary: Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins. Has exodeubiquitinase activity and has a preference for long polyubiquitin chains. May play a regulatory role at the level of protein turnover.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).