5830415F09Rik (BC092543) Mouse Untagged Clone

CAT#: MC218474

5830415F09Rik (untagged) - Mouse RIKEN cDNA 5830415F09 gene (cDNA clone MGC:107342 IMAGE:30315335), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 5830415F09Rik
• Synonyms: 5830415F09Rik; AV014846; Nap1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC092543
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGGCTTGGAGAAGCAGGGCTCGTGTGCTACGGCGGCCCCATGTGGGTGCGTTCAGCCGGTCCTGG
  AGGCAGGGAATCTTTTAACTGAGCCAATAGGGTACTTGGAATCATGTTTCCCAGCCAAGATTGGCACTCC
  AAGGCAGCCATCCATCTGTAGCCACTCCAGAGCCTGTTTGAAGATCAGAAAGAACATCTTTAATAACCCT
  GAGCATTCCTTGATGGGCCTAGAAGAGTTTTCTCATGTTTGGATTTTGTTTGTTTTTCACAAAAATGGCC
  ATTTGAACTACAAGGCAAAAGTGCAGCCGCCCAGGCTGAATGGGGCAAAGACTGGAGTGTTCTCCACACG
  GAGCCCTCATCGCCCTAATGCCATAGGGCTGACGCTGGCCAAGCTGGAGAAGGTGGAAGGTGGAGCTGTC
  TACCTGTCTGGAGTTGACATGATAGACGGCACACCTGTGCTAGACATAAAGCCCTACATTGCTGACTACG
  ACTCACCACAGAACCTCAGTGTACACAATGACCACCATAAGCTGAGGGCTGAGGCCCAGGTTGATGGCAC
  GGCCAACAGTTGTGACCAGCTGCTGCTCTCAGGGCGTGGGAAAGTACAGCCTCGCCAGAGCACCAAGGAG
  AGACCGAAATGCCTTGAAGACAGGACCTCAGGAGAAAACTCTCAGAAGTCCAGAGACATGTCAGAAATCC
  AGCACACTTTGCCAGAAGACAGAGAGAGAGCGTTGGATCTGGCACTGGAACCAAGCCGAGGTGAGAGCAT
  GGATATGCCCGAAAATCAGCTTGGCCCACCGGAACTGAAGAGCTTTCTGGAAGAAGGCACAGATAGACCA
  AGGAAAGTAGAAGGTGCCTTGGTCCTGCCGGGGAGCAGTGCAGAAACACAGTGGGATGCTTCCTACCGTG
  CCAGGACAGCTGACAGAGTGCCCTACAGTGTTGTCCCCAGCTGGGTGACAGAGGCTCCTGTAGCCCCTTT
  ACAAGTCCGGTTTACTCCTCACGCAGAGATGGATCTGAGGAAGCTCAATTCAGGAGATGCTAGTCAGCCG
  TCATTTAAGTACTTCCACTCCGCAGAGGAAGCCAAGCGTGCCATTGAGGCTGTGCTGTCAGCAGATCCTC
  GGTCTGTGTACCGCCGGAAGCTCTGCGAGGACCGCCTTTTCTTCTTCACTGTGGACACAGCACATGTCAC
  CTGCTGGTTTGGTGATGGCTTCGCAGAGGTTGTGAGGATTAAACTGGCTTCTGAGTCTGTTCAGGTGGCT
  GACCCTGAGGAATCCTTGGCGGCCCTGGGGTCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC092543
• Insert Size: 1296 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC092543, AAH92543
• RefSeq Size: 1561 bp
• RefSeq ORF: 1295 bp
• Locus ID: 74753
• Cytogenetics: 4 B1
• Gene Summary: S-adenosyl-L-methionine-dependent methyltransferase responsible for the addition of the methyl group in the formation of N6-methyl-N6-threonylcarbamoyladenosine at position 37 (m(6)t(6)A37) of the tRNA anticodon loop of tRNA(Ser)(GCU). The methyl group of m(6)t(6)A37 may improve the efficiency of the tRNA decoding ability. May bind to tRNA.[UniProtKB/Swiss-Prot Function]