Otud6b (BC031474) Mouse Untagged Clone

CAT#: MC218444

Otud6b (untagged) - Mouse OTU domain containing 6B (cDNA clone MGC:27712 IMAGE:2599116), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Otud6b
• Synonyms: 2600013N14Rik; AU015433
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC031474
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGGTCGTGGCTGAAGAGCTCGATGATGAGGAGCAGCTGGTGAGAAGGCATCGGAAGGAGAAGA
  AGGAGCTGCAAGCCAAAATTCAGGGAATGAAGAACGCTGTTCCCAAAAACGACAAAAAGAGGAGGAAGCA
  GCTCACGGAAGATGTTGCTAAATTGGAAAGAGAAATGGAGCAAAAACACAGGGAAGAACTGGAGCAATTG
  AAGCAATTGACTTTCAAGGACAGTAAGATAGATTCTGTTGCTGTTAACATTTCAAACTTGGTACTTGAGA
  ATCAACCACCTCGGATTTCAAAAGCACAAAAGAGACGGGAAAAGAAGGCTGCATTGGAAAAGGAGCGGGA
  AGAAAGGATAGCAGAGGCTGAAATTGAGAACTTATCTGGAGCCAGACATCTTGAGAGTGAAAAACTTGCT
  CAAATATTGGCAGCCAGAGAATTGGAAATTAAACAGATTCCATCTGATGGTCACTGTATGTATGGAGCAC
  TTGAGGATCAGCTGAGAGAACAAGACTGTGCGCTGACTGTGGCTTCCCTGAGGAGGCAGACTGCTGAGTA
  CATGCAAACCCATTCAGACGACTTCCTGCCGTTTCTAACAAACCCCAGCACAGGAGATATGTACACGCCA
  GAAGAGTTTGGGAAGTACTGTGACGATATTGTGAACACAGCGGCATGGGGCGGTCAGCTCGAGCTAAGAG
  CTTTGTCTCACATCTTACAAACACCAATCGAGATACTACAGGCAGATGCTCCTCCTATTATAGTCGGTGA
  AGAGTATCCGAGGAATCCATTAGTACTAGTATACATGAGGCACGCGTATGGCTTAGGAGAACATTATAAT
  TCTGTTACACGGTTGGTGAACTCAGCTACTGAAAATTGCAGCTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATA
  TCCTGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: BC031474
• Insert Size: 885 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031474, AAH31474
• RefSeq Size: 2598 bp
• RefSeq ORF: 884 bp
• Locus ID: 72201
• Cytogenetics: 4 A1
• Gene Summary: Deubiquitinating enzyme that may play a role in the ubiquitin-dependent regulation of protein synthesis, downstream of mTORC1 (By similarity). May associate with the protein synthesis initiation complex and modify its ubiquitination to repress translation (By similarity). May also repress DNA synthesis and modify different cellular targets thereby regulating cell growth and proliferation (By similarity). May also play a role in proteasome assembly and function (By similarity).[UniProtKB/Swiss-Prot Function]