Ddx28 (NM_028038) Mouse Untagged Clone

CAT#: MC218437

Ddx28 (untagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 28 (Ddx28), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ddx28
• Synonyms: 2410004K13Rik; AI449652; Mddx28
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218437 representing NM_028038
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGCTTTAGCCGGGCCATCGCGGCTGCTGGCGTTAGCAGTACGGTTGCTTCTGGAGCCTCGACGGAACC
  TAGTGGTGCGCGGTTCCGACCAGTCCTTGCCAGTGGTGCGCGTTCCGCGGGCTCTGCAGCGACGGCAGGA
  ACAGCGACAGAGCGGCCGTGGGAGTTTGCAGCGGCCAGTGCTAGTGCGCCCCGGTCCCCTGCTGGTCTCG
  GCGCGGCGGCCGGAGTTGAACCAGCCCGCACGCCTCACGCTGGGCCGTTGGGAGCGAGCGCCACTGGCTT
  CCCGTGGCTGGAAGCATCGGCGATCGCGGCAAGACCATTTCTCCATAGAGCGAGTGCAGCAAGAGGCGCC
  CGCCCTGAGGAACCTCTCGTCCCGGGGCAGCTTCGTGGACCTGGGTCTGGAACCCCGAGTGCTGCTGGCG
  CTTCAGGAGGCTGTCCCCGAAGTTGTGCAGCCAACCTCAGTGCAGTCGAAAACCATCCCCCCACTGCTTC
  GAGGCCGCCACCTCCTTTGCGCTGCGGAAACTGGCAGTGGCAAGACGCTTAGCTACCTACTGCCCCTATT
  TCAGCGGCTCCTGAGAGGGTCAGACCTGGACTCCCGCAGTTTCACGGCTCCTCGAGGCTTGGTTCTGGTA
  CCTTCCCGAGAACTAGCCGAACAGGTGCAGGCAGTGGCCCAGTCACTGGGTGGGTACTTGGGCCTGCAGG
  TGATAGAACTTGGCGGAGGCCTCGGGATGAGTAGGCTCAAACTTCAGCTGTATAGACGACCGGCAGCCGA
  TGTGTTAGTGGCTACTCCAGGGGCGCTGTGGAAGGCTCTGAAAAGTCAACTGATCAGCTTACAGCATCTT
  AACTTCATAGTGCTAGATGAAGTAGACACACTGCTAGATGAAAGCTTCCTGGAACTGGTGGACTACATCT
  TGGAAAAGAGCCCTATAGCAGAAAGTCCAGCCGAGTTAGAAGACCCCTTTAATCCCAAAGCCCAGTTAGT
  GCTGGTGGGAGCCACGTTTCCAGAAGGCCTAAACCAGTTACTGAGTAAAGTCACCAGTCCAGACAGTCTG
  ACCACCATTACCAGCTCCAAGCTGCACTGTCTCATGCCTCATGTCAGACAGACATTTATGAGACTGAAGG
  GAGCAGACAAGGTGACAGAACTGGTTCAGATCCTCAAACAGCAGGACAAAGCAAGTAAGACGGAACCCTC
  GGGAACTGTCCTGGTATTCTGCAACAGCGCCAGCACCGTGAACTGGCTTGGGTACATTCTGGATGACCAC
  AAGATTCAACATCTAAGGCTGCAAGGGCAAATGCCGGCCTCAATGAGGGCAGGTATCTTCCAGAGTTTCC
  AGAAGGGCTCCCAGAACATACTTGTCTGCACAGACATCGCCTCCCGAGGCCTAGACAGTGTCCACGTGGA
  AGTGGTTATCAATTACGATTTCCCCCCTACCCTGCAGGATTACATCCACAGAGCAGGAAGGGTGGGTCGT
  GTGGGCAGCGAAGTGCCAGGGTCCGTTATCAGTTTTGTGACCCACCCCTGGGATGTGAGCCTGGTTCAGA
  AAATTGAACTGGCAGCTCGCCGAAGGAGAAGCCTTCCAGGACTAGCATCTTCAGTGGGGGACCCTTTGCC
  TCAAAAGGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: AscI-MluI
• ACCN: NM_028038
• Insert Size: 1623 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028038.3, NP_082314.2
• RefSeq Size: 1871 bp
• RefSeq ORF: 1623 bp
• Locus ID: 71986
• UniProt ID: Q9CWT6
• Cytogenetics: 8 D3
• Gene Summary: Plays an essential role in facilitating the proper assembly of the mitochondrial large ribosomal subunit and its helicase activity is essential for this function. May be involved in RNA processing or transport. Has RNA and Mg(2+)-dependent ATPase activity (By similarity).[UniProtKB/Swiss-Prot Function]