E130307M08Rik (BC017625) Mouse Untagged Clone

CAT#: MC218396

E130307M08Rik (untagged) - Mouse RIKEN cDNA E130307M08 gene (cDNA clone MGC:28784 IMAGE:4488477), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: E130307M08Rik
• Synonyms: E130307M08Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC017625
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTAGGGGGCGCGCTCACGAGGCGCGCGGTCACGCTTCGGGTGCTGCTCAAAGACGAGCTGCTGG
  AGCCCGGTGAGGGGGTGCTATCCATCTACTACCTGGGCAGGAAGTTTACTGGGGACCTGCAGCTGGATGG
  CAGGATCGTGTGGCAGGAAACTGGACAAGTCTTCAACTCACCCAGCGCCTGGGCCACACACTGCAAGAAG
  CTCGTCAACCCAGCCAAGAAATCTGGCTGTGGCTGGGCCTCTGTCAAGTACAAGGGCCAGAAGCTGGACA
  AATACAAGGCCGCCTGGCTTCGCCGGCACCAGCTACATATGCCTGTAGCTACGGCCGATGAGAGTCCCAC
  CAGTGAAGGGGAGGAGGAAGAACTGCTGTTGGAGGAGGAGGAAGAAGATGTGCTGGCTGGGGTCTCATCA
  GAGGACAAAGGCCACAGACCCCCTGGGAAGGGTTCCTTGGAGCCAGAGGCCACACCCCCAGGGAAGCGTA
  TGGACAAGGTGCCAGTGCCCATCCGTTACTGCATGCTGGGCAGCCGAGACTCCGCCAGGAACCCCCACAC
  TCTGGTAGAAGTAACATCCTTTGCTGCCATCAACAAGTTCCAGCCATTCAACGTGGCCGTTTCCAGTAAT
  GTGCTGTTCCTCTTGGACTTCCACTGTCACCTGACTCGGAGTGAGGTCGTGGGCTACCTTGGTGGTCGCT
  GGGACATCAACAATCAGATGCTGACCGTGCTGAGAGCCTTCCCCTGCAGGAGTCGGCTGGGGGACACTGA
  TACTGCAGCCACGGTTGAGGAGGAGATCTACCAGGTCCTGTTCCTTCGAGGCCTGTCCCTGGTGGGCTGG
  TACCACAGCCACCCACACAGCCCTGCAGTACCCTCCTTGCAGGACATTGACGCACAGATGGAGTACCAGT
  TGAGACTGCAAGGCTCCAGCAATGGCTTCCAGCCCTGCCTGGCCCTGCTGTGCTCCCCCTACTACTCTGG
  CAACCCAGGCCCTGAGTCCAAGATCTGCCCTTTCTGGGTGATGCCTCCCCCTGAGCAAAGGCCCAGTGAC
  TATGGAATCCCCATGGACGTAGAGATGGCGTATGTCCAAGATAGCTTCTTGACCAACGATGTTCTTCAGG
  AGATGGTGATGCTGGCTGAGTTCTACAAGGGTGCCCCTGACCTGGTGAAGTTCCAAGAGGCCTGGAGCCC
  AGAGCATACCTACCTGGACAAACTCAAGATGTCCTTGGCCAGCAGGACCCCGAAGGACCAGGGCATGTGC
  CACGTGCTGGAGCAGGTCTGCAGTGTGCTCAAGCAGGGGAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC017625
• Insert Size: 714 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC017625, AAH17625
• RefSeq Size: 1538 bp
• RefSeq ORF: 1304 bp
• Locus ID: 68047
• Cytogenetics: 17 D
• Gene Summary: Probable protease.[UniProtKB/Swiss-Prot Function]