Nudt12 (BC057657) Mouse Untagged Clone

CAT#: MC218395

Nudt12 (untagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 12 (cDNA clone MGC:68086 IMAGE:2646355), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nudt12
• Synonyms: 0610016O18Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC057657
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCTGTTAAAAGAAATCCCAAAAAGGAGATGATTTCTGAACTCCACAGTTCAGCCGCAGAGGGAA
  ATGTCGCAAAGTTAGCAGGAATACTCAGCCATTCTCCATCTCTTCTCAATGAGACTTCTGAAAATGGATG
  GACTGCTTTAATGTACGCCGCCAGGAACGGGCATCCCGATGTGGTCCAGTTTCTGCTTGAGAAAGGATGT
  GACAGATCACTTGTCAATAAAGGGAGGCAAACCGCCCTGGATATTGCTGCATTTTGGGGTTATAGGCATA
  TAGCTAACTTGTTAGCAAATGCAAAAGGTGGGAAGAAGCCCTGGTTCCTAACCAATGAAGTAGACGAGTG
  TGAGAATTATTTTAGCAGAACACTACTGGACCGGAGGAGTGACAAAAGAAATAATTCTGACTGGCTGCAA
  GCTAAAGAGAGCCACCCCACCACAGTTTATCTCCTTTTCTCAGACTTGAACCCCCTGGTTACCCTAGGTG
  GTAATAAAGAAAGCTCGCAGCAGCCGGAAGTCCGGCTTTGCCAGCTGAACTACCCGGATGTAAAGGGTTA
  CTTGGCTCAGCCTGAGAAGATCACCTTGGTGTTCCTTGGAGTCGAGCTTGAGATGAGGAAAGGCTCACCC
  GCCCAGGCGGGAGGAGTCCCAGAGGAAGAGGAGGACGGTTTGGTTGCTTGGTTTGCCCTCGGTATTGAAC
  CCGGTGCTGCTGAGGAGTTTAAGCAAAGACATGAAAATTGTTATTTTCTCCACCCGCCAATGCCAGCTCT
  TCTGCAGTTGAAAGAAAAGGAGGCTGGGGTGGTAGCTCAAGCAAGATCAGTGCTTGCCTGGCATAGTCGA
  TACAAGTTCTGCCCAACCTGTGGCAGTGTGACTAAAATTGAGGAAGGAGGCTACAAAAGAGTGTGTGTAC
  GAGAGACTTGCCCTAGTCTCCAAGGCGTCCACAACACATCTTACCCGAGAGTTGATCCAGTCGTAATTAT
  GCAGGTCATCCATCCAGATGGAACCAAATGTCTTCTAGGCAGGCAAAAGCGATTTCCCCCGGGCATGTTC
  ACATGTCTTGCTGGATTTATAGAGCCCGGTAAGCCCATACTGACTGGTTTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC057657
• Insert Size: 1104 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057657, AAH57657
• RefSeq Size: 1299 bp
• RefSeq ORF: 1103 bp
• Locus ID: 67993
• Cytogenetics: 17 D
• Gene Summary: Hydrolyzes NAD(P)H to NMNH and AMP (2',5'-ADP), and diadenosine diphosphate to AMP. Has also activity towards NAD(P)(+), ADP-ribose and diadenosine triphosphate. May act to regulate the concentration of peroxisomal nicotinamide nucleotide cofactors required for oxidative metabolism in this organelle (By similarity).[UniProtKB/Swiss-Prot Function]