Ift74 (BC049156) Mouse Untagged Clone

CAT#: MC218386

Ift74 (untagged) - Mouse intraflagellar transport 74 homolog (Chlamydomonas) (cDNA clone MGC:56922 IMAGE:6313441), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ift74
• Synonyms: 1700029H06Rik; b2b796Clo; Ccdc2; Cmg1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC049156
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGTAATCACAAATCTTCAGCCCCTCGCCCTATTTCTCGGGGTGGAATAGGACTAACAGGAAGGC
  CTCCTTCTGGAATAAGACCTCCATCTGGCAATGTTCGAGTGGCAACTGCAATGCCACCAACAACAGCAAG
  ACCAGGTTCTCGTGGTGGTCCCTTAGGGACTGGTGGAGTTTTGTCATCTCAAATCAAAGTTGCTGATCGT
  CCTGTGACCCAACAAGGTTTGAGTGGAATGAAGACTGGCATGAAAGGTCCCCAGAGGCAAATTTTAGACA
  AATCTTACTATCTTGGACTTCTTAGGAGCAAAATAAGTGAACTTACAACTGAAATTAATAAGCTTCAGAA
  AGAAATAGAAATGTACAACCAAGAAAATTCAGTGTATTTGTCATATGAAAAGAGGGCAGAGACATTAGCT
  GTTGAAATTAAAGACTTTCAAGGACAACTAGCAGACTACAACATGTTGGTAGATAAACTTAATACCAATA
  CTGAAATGGAAGAAGTAATGAGTGATTACAACATGCTTAAAGCTCAAAATGACAGAGAAACACAAAGTAT
  GGATGTCATATTTACTGAAAGACAAGCGAAGGAAAAACAAATCAGAAGTGTAGAAGAAGAAGTTGAACAA
  GAAAAGCAAGCAGCAGATGGCATTATCAAAAATATGTCTCCTGAAAAACAAGTCAAGTACATAGAAATGA
  AAACCACAAATGAGAAACTGTTGCAGGAATTAGATACACTTCAGCAACAGCTAGATTCTCTGAACATGAA
  AAAGGAGAGCCTAGAAACTGAAATAGCACACTCCCAAGTAAAACAAGAGGCTGTATTGCTGTATGAAAAA
  CTTTATGAGTTAGAGTCCCATCGAGATCAAATGATTGCAGAAGACAAAAGCATGGGATCGCCAATGGAGG
  AAAGGGAGAGATTGTTTAAGCAGGTTAAAGAAGATAATCAGGAAATAGCCAGCATGGAGAGACAGTTAAC
  AGATATAAAAGAAAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC049156
• Insert Size: 1607 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC049156
• RefSeq Size: 1659 bp
• RefSeq ORF: 998 bp
• Locus ID: 67694
• Cytogenetics: 4 C5
• Gene Summary: Component of the intraflagellar transport (IFT) complex B: together with IFT81, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds beta-tubulin via its basic region. Required for ciliogenesis (By similarity).[UniProtKB/Swiss-Prot Function]