Treh (BC019214) Mouse Untagged Clone

CAT#: MC218346

Treh (untagged) - Mouse trehalase (brush-border membrane glycoprotein) (cDNA clone MGC:29262 IMAGE:5064708), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Treh
• Synonyms: 2210412M19Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC019214
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCTGGGAGCTGCACCTGCTGCTTCTGCTGGGGCTGGGACTTAGGTCCCAGGAGGCCCTGCCACCAC
  CCTGTGAGAGATCTACTGCCATGGAGAGCTCCTGCACCAAGTTCAGATGGCCCAGCTCTACCAAGATGAC
  AAGCAGTTTGTGGATATGTCACTGGCCACATCTCCAGATGAAGTCCTGCAGAAGTTCAGTGAGCTGGCCA
  CAGTCCACAACCACAGCATCCCCAAGGAACAGCTTCAGGAATTTGTCCAGAGTCACTTCCAGCCCGTGGG
  GCAGGAGCTGCAGTCCTGGACCCCTGAGGACTGGAAGGACAGCCCTCAGTTCCTGCAGAAGATCTCGGAT
  GCTAATCTGCGTGTCTGGGCGGAGGAGCTACACAAGATCTGGAAAAAGCTGGGAAAGAAGGGACTCGTAC
  TGGGTGATGGAAGGCCTGCTTCTTTCTGAGATGGCCTCAACAGTGAAGGGTATGCTGCAGAACTTTCTGG
  ATCTGGTGAAGACCTACGGACATATCCCCAATGGTGGACGCATATATTACCTGCAACGGAGCCAGCCCCC
  ACTCCTGACTCTCATGATGGATCGATATGTAGCTCATACCAAGGATGTCGCCTTCCTTCAGGAGAATATT
  GGGACTCTAGCCTCTGAACTGGACTTCTGGACTGTGAACAGGACTGTCTCTGTAGTCTCAGGAGGACAAA
  GCTATGTCTTAAATCGCTACTATGTCCCTTATGGGGGACCCAGGCCAGAGTCCTACAGGAAAGACGCAGA
  ATTGGCAAACTCTGTGCCAGAAGGGGACCGAGAGACTCTGTGGGCTGAGCTCAAGGCTGGGGCTGAGTCT
  GGCTGGGACTTCTCTTCACGCTGGCTTGTTGGAGGCCCAGACCCTGATTTGCTCAGCAGCATCCGAACCA
  GCAAAATGGTACCCGCTGATCTGAACGCGTTCCTGTGCCAAGCAGAGGAACTGATGAGTAACTTCTACTC
  CAGACTAGGGAACAACACAGAGGCCACAAAGTACAGGAACCTGCGGGCCCAGCGCTTGGCCGCCATGGAA
  GCTGTCCTGTGGGACGAGCAGAAGGGTGCCTGGTTCGACTATGACTTGGAAAAGGGGAAGAAGAACCTGG
  AGTTTTATCCCTCCAACCTCACCCCACTTTGGGCTGGCTGCTTCTCAGACCCTAGTGTTGCTGACAAGGC
  TCTGAAGTACTTGGAGGACAGCAAGATCTTGACCTACCAATATGGAATCCCAACCTCTCTTCGTAACACA
  GGCCAGCAGTGGGACTTCCCCAATGCCTGGGCCCCACTGCAGGACCTGGTCATTAGAGGTTTGGCCAAGT
  CAGCTTCCCCCCGGACTCAGGAGGTGGCTTTCCAGCTGGCCCAGAATTGGATCAAAACCAACTTCAAAGT
  CTACTCCCAAAAGTCAGCGATGTTTGAGAAGTATGACATCAGCAACGGTGGACATCCAGGTGGAGGAGGG
  GAGTATGAAGTTCAGGAAGGATTTGGCTGGACAAACGGATTGGCCCTGATGCTTCTGGATCGCTATGGTG
  ACCAGTTGACTTCAGGGACCCAGTTAGCTTCCCTGGGACCCCACTGCCTAGTGGCTGCCCTTCTTCTCAG
  TCTTCTGCTACAGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATA
  TCCTGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: BC019214
• Insert Size: 1626 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC019214, AAH19214
• RefSeq Size: 1972 bp
• RefSeq ORF: 1625 bp
• Locus ID: 58866
• Cytogenetics: 9 A5.2
• Gene Summary: This gene belongs to the alpha-glucosidase family, whose members encode enzymes that carry out hydrolysis of alpha-glucoside bonds of a variety of carbohydrates. The enzyme encoded by this gene uses the disaccharide trehalose as a highly specific substrate and converts it into two glucose molecules. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2013]