Amot (BC057638) Mouse Untagged Clone

CAT#: MC218292

Amot (untagged) - Mouse angiomotin (cDNA clone MGC:67773 IMAGE:2631191), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Amot
• Synonyms: CAG-2, mKIAA1071
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC057638
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACACCGAGGTCCCCCGCCAGAATACCCTTTCAAGGGCGTGCCATCCCAGTCTGTAGTGTGCAAGT
  CCCAAGAGCCAGGGCACTTCTATAGTGAGCATCGCCTGAACCAGCCAGGGAGAACAGAGGGGCAACTGAT
  GAGGTATCAGCACCCCCCTGAGTATGGGGCAGCCAGGGCAACACAGGACATCTCCTCGCTGTCTTTGTCA
  GCCAGGAACTCTCAACCTCATAGCCCTACTTCTTCCCTGACAGCTGGGGCTTCCAGCTTGCCCTTGCTGC
  AGTCTCCGCCGTCCACTAGATTGCCTCCTGGTCAACACCTGGTCCCGAACCAAGGTGACCACTCTGCTCA
  CCTGTCAAGGCATCAGCAGCATCTTCTTTCGAGTCAGAGTCATCAGGGGGACCACTACCGTCACGCCCAG
  GCCAGCCTGACTTCAGCGCAGCAGCAGCCAGGAGAAGCCTACTCAGCTATGCCTCGGGCTCAGCAGTCTG
  CTTCTTATCAGCCCATGCCAGCTGACCCTTTTGCTATCGTCTCTAGAGCTCAGCAGATGGTTGAGATCCT
  GTCTGATGAGAATCGCAACTTGCGGCAGGAGTTGGATGGATGCTATGAGAAGGTGGCGAGACTGCAGAAG
  GTGGAGACAGAAATCCAACGGGTCTCAGAGGCTTATGAGAACTTGGTGAAGTCATCTTCCAAAAGAGAGG
  CTCTGGAGAAAGCCATGAGGAACAAGCTGGAGGGCGAGATTAGAAGGATGCATGACTTCAACAGAGATCT
  GAGAGACCGTCTAGAGACTGCCAACAAGCAGCTGGCAGAGAAGGAGTACGAGGGGTCCGAGGACACCAGG
  AAGACCATCTCGCAGCTCTTTGCCAAACATAAAGAAAACCAGCGGGAGAAGGAGAAGCTGGAAGCTGAGC
  TGGCTACTGCCCGTTCTACCAATGAAGACCAAAGGCGACACATTGAAATCCGAGACCAGGCATTGAGCAA
  TGCCCAGGCGAAGGTGGTGAAGCTGGAAGAAGAGCTGAAAAAGAAGCAAGTGTACGTAGATAAGGTGGAG
  AAGATGCAGCAGGCCCTTGTGCAGCTCCAGGCCGCCTGTGAGAAGCGTGAGCAGCTGGAACACCGGCTCC
  GGACACGTTTGGAGAGGGAACTGGAATCCCTTAGAATCCAGCAGCGCCAGGGCAACTCCCAGCCCACTAA
  TGCTTCAGAATACAATGCCGCTGCACTGATGGAGCTCCTCCGGGAGAAGGAAGAGAGAATTCTGGCCCTG
  GAAGCAGATATGACCAAGTGGGAGCAAAAGTATTTGGAAGAGAATGTGATGAGACACTTTGCTCTGGATG
  CTGCTGCAACTGTGGCTGCACAGAGAGACACAACAGTCATTAGCCACTCTCCTAACACCAGCTATGACAC
  AGCCTTAGAGGCCCGCATCCAGAAGGAAGAGGAAGAAATCCTGATGGCCAACAAGCGTTGCTTGGACATG
  GAAGGAAGGATTAAGACCCTCCATGCCCAGATTATTGAAAAGGATGCCATGATCAAGGTGCTGCAGCAGC
  GCTCCAGAAAGGAGCCGAGTAAAACAGAGCAACTCTCATCCATGCGGCCAGCAAAGTCTCTGATGTCCAT
  TTCCAATGCTGGATCAGGCCTCCTTGCATACTCCTCTACCTTGACTGGTGCGCCCATTATGGAGGAGAAG
  CGTGATGACAAGAGCTGGAAGGGGAGCCTAGGTAAGGAAAAGGAAAGCAACAGAAGCAAAGGCACAGTGA
  CAGACTTAGAAAGTGTCCTGACTCTGTTACACACAGCAAGGAAAAGGGACAATGGTCCAGGTTCTAGAGA
  GGAGAATTTGGAGTCCCCCCTCTCAATGGAATTAGATTTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC057638
• Insert Size: 1863 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057638, AAH57638
• RefSeq Size: 2405 bp
• RefSeq ORF: 1862 bp
• Locus ID: 27494
• Cytogenetics: X F2
• Gene Summary: Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions (By similarity).[UniProtKB/Swiss-Prot Function]