Macroh2a1 (NM_001159515) Mouse Untagged Clone

CAT#: MC218287

H2afy (untagged) - Mouse H2A histone family, member Y (H2afy), transcript variant 4, (10ug)


  "NM_001159515" in other vectors (3)

Reconstitution Protocol

USD 503.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Macroh2a1
Synonyms H2af; H2AF12; H2AF12M; H2afy; MACROH2; mH2a; mH2a1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC218287 representing NM_001159515
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGAGCCGCGGCGGGAAGAAGAAATCCACCAAGACCTCCCGGTCAGCCAAGGCCGGAGTCATCTTCC
CTGTGGGACGCATGCTTCGGTACATCAAGAAAGGCCACCCTAAGTATAGGATCGGAGTGGGGGCACCTGT
GTACATGGCTGCTGTCCTGGAGTACCTGACTGCTGAGATCCTGGAGCTGGCTGGCAATGCAGCAAGAGAC
AACAAGAAGGGACGGGTCACACCCCGGCACATCCTGTTAGCTGTGGCCAATGATGAAGAGCTAAACCAGC
TGCTAAAGGGTGTCACCATAGCCAGCGGGGGCGTGTTGCCGAATATCCATCCTGAGTTGCTAGCGAAGAA
GCGAGGATCCAAGGGAAAATTGGAAGCCATCATCACGCCTCCGCCGGCCAAAAAGGCCAAGTCTCCATCC
CAGAAGAAGCCAGTGGCTAAGAAGACAGGAGGCAAGAAAGGGGCCCGGAAGTCTAAGAAGCAGGGAGAAG
TGAGCAAGGCGGCCAGCGCAGACAGTACGACGGAGGGCACGCCTACAGACGGCTTCACTGTCCTCTCCAC
CAAGAGCCTCTTCCTCGGCCAGAAGTTGCAAGTTGTTCAGGCTGACATTGCCTCGATCGACAGTGATGCT
GTCGTTCACCCGACAAACACTGACTTCTACACCGGTGGTGAAGTAGGAAACACACTGGAGAAGAAGGGCG
GCAAGGAGTTTGTAGAAGCTGTTCTGGAACTCCGGAAAAAGAACGGGCCCTTGGAGGTAGCTGGAGCTGC
TATTAGTGCAGGCCATGGCCTGCCTGCCAAGTTTGTGATCCACTGTAATAGTCCTGTCTGGGGTGCAGAC
AAATGTGAAGAACTTCTAGAAAAGACGGTGAAAAACTGCTTGGCTCTAGCTGATGACAGAAAGCTGAAAT
CCATCGCCTTCCCATCCATTGGCAGCGGCAGGAACGGGTTCCCGAAGCAGACAGCGGCCCAGCTCATTCT
GAAGGCCATCTCCAGCTACTTTGTCTCCACGATGTCCTCCTCCATCAAAACTGTGTACTTCATGCTTTTT
GACAGTGAGAGCATAGGTATCTATGTGCAGGAAATGGCCAAGCTGGACGCCAACTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001159515
Insert Size 1107 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001159515.1, NP_001152987.1
RefSeq Size 1966 bp
RefSeq ORF 1107 bp
Locus ID 26914
UniProt ID Q9QZQ8
Cytogenetics 13 B1
Gene Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2015]
Transcript Variant: This variant (4) uses an alternate in-frame splice site and an alternate exon in the central coding region, compared to variant 1. The resulting isoform (4) lacks an internal residue and differs in an internal segment, compared to isoform 1.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.