Map3k7 (NM_009316) Mouse Untagged Clone

CAT#: MC218284

Map3k7 (untagged) - Mouse mitogen-activated protein kinase kinase kinase 7 (Map3k7), transcript variant B, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Map3k7
• Synonyms: B430101B05; C87327; Tak1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218284 representing NM_009316
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGACAGCCTCCGCCGCCTCGTCCTCCTCCTCGTCTTCTGCCAGTGAGATGATCGAAGCGCCGTCGC
  AGGTCCTGAACTTCGAAGAGATCGACTACAAGGAGATCGAGGTGGAAGAGGTTGTCGGAAGAGGAGCTTT
  TGGAGTAGTTTGCAAAGCTAAGTGGAGAGCAAAAGATGTCGCTATTAAACAGATAGAAAGTGAGTCTGAG
  AGGAAGGCTTTCATTGTGGAGCTCCGGCAGTTGTCACGTGTGAACCATCCTAACATTGTCAAGTTGTATG
  GAGCCTGCCTGAATCCAGTATGTCTTGTGATGGAATATGCAGAGGGGGGCTCATTGTATAATGTGCTGCA
  TGGTGCTGAACCATTGCCTTACTACACTGCTGCTCATGCCATGAGCTGGTGTTTACAGTGTTCCCAAGGA
  GTGGCTTACCTGCACAGCATGCAGCCCAAAGCGCTGATTCACAGGGACCTCAAGCCTCCAAACTTGCTGC
  TGGTTGCAGGAGGGACAGTTCTAAAAATCTGCGATTTTGGTACAGCTTGTGACATCCAAACACACATGAC
  CAATAATAAAGGGAGTGCTGCTTGGATGGCGCCTGAAGTATTTGAAGGTAGCAATTACAGTGAAAAGTGT
  GATGTCTTCAGCTGGGGTATTATCCTCTGGGAAGTGATAACACGCCGGAAACCCTTCGATGAGATCGGTG
  GCCCAGCTTTCAGAATCATGTGGGCTGTTCATAATGGCACTCGACCACCACTGATCAAAAATTTACCTAA
  GCCCATTGAGAGCTTGATGACACGCTGTTGGTCTAAGGACCCATCTCAGCGCCCTTCAATGGAGGAAATT
  GTGAAAATAATGACTCACTTGATGCGGTACTTCCCAGGAGCGGATGAGCCGTTACAGTATCCTTGTCAGT
  ACTCTGATGAAGGGCAGAGCAACTCAGCCACCAGCACAGGCTCATTCATGGACATTGCTTCTACAAATAC
  CAGTAATAAAAGTGACACAAATATGGAACAGGTTCCTGCCACAAACGACACTATTAAACGCTTGGAGTCA
  AAACTTTTGAAAAACCAGGCAAAGCAACAGAGTGAATCTGGACGCCTGAGCTTGGGAGCCTCTCGTGGGA
  GCAGTGTGGAGAGCTTGCCCCCCACTTCCGAGGGCAAGAGGATGAGTGCTGACATGTCTGAAATAGAAGC
  CAGGATCGTGGCGACTGCAGCCTATTCCAAGCCTAAACGGGGCCACCGTAAAACCGCTTCATTTGGCAAC
  ATTCTGGATGTCCCTGAGATCGTCATATCAGGTAACGGGCAACCAAGGCGTAGATCCATCCAAGACTTGA
  CTGTTACTGGGACAGAACCTGGTCAGGTGAGCAGCCGGTCATCCAGCCCTAGTGTCAGAATGATCACTAC
  CTCAGGACCAACCTCAGAGAAGCCAGCTCGCAGTCACCCGTGGACCCCTGATGATTCCACAGATACCAAT
  GGCTCAGATAACTCCATCCCAATGGCGTATCTTACACTGGATCACCAGCTACAGCCTCTAGCGCCGTGCC
  CAAACTCCAAAGAATCCATGGCAGTGTTCGAACAACATTGTAAAATGGCACAGGAGTATATGAAAGTTCA
  AACCGAAATCGCATTGTTACTACAGAGAAAGCAAGAACTAGTTGCAGAATTGGACCAGGATGAAAAGGAC
  CAGCAAAATACATCTCGTCTGGTACAGGAACATAAAAAGCTTTTAGATGAAAACAAAAGCCTTTCTACTT
  ATTACCAGCAATGCAAAAAACAACTAGAGGTCATCAGAAGCCAACAGCAGAAACGACAAGGCACTTCATG
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009316
• Insert Size: 1821 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009316.1, NP_033342.1
• RefSeq Size: 5763 bp
• RefSeq ORF: 1821 bp
• Locus ID: 26409
• Cytogenetics: 4 A5
• Gene Summary: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR) (PubMed:10748100, PubMed:16157589, PubMed:21183079, PubMed:29291351). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs, c-jun N-terminal kinases (JNKs) and I-kappa-B kinase complex (IKK). Both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1), while nuclear factor-kappa B is activated by IKK (PubMed:16157589, PubMed:8533096, PubMed:29291351). MAP3K7 activates also IKBKB and MAPK8/JNK1 in response to TRAF6 signaling and mediates BMP2-induced apoptosis (PubMed:10748100). In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK1, but not that of NF-kappa-B. Promotes TRIM5 capsid-specific restriction activity (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (B) represents the longer transcript and encodes the longer isoform (B). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.