Tgfbr2 (BC052629) Mouse Untagged Clone

CAT#: MC218257

Tgfbr2 (untagged) - Mouse transforming growth factor, beta receptor II (cDNA clone MGC:54755 IMAGE:6310023), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tgfbr2
• Synonyms: DNIIR, RIIDN, TbetaRII, TbetaR-II
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052629
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCGGGGGCTGCTCCGGGGCCTGTGGCCGCTGCATATCGTCCTGTGGACGCGCATCGCCAGCACGA
  TCCCGCCGCACGTTCCCAAGTCGGATGTGGAAATGGAAGCCCAGAAAGATGCATCCATCCACCTAAGCTG
  TAATAGGACCATCCATCCACTGAAACATTTTAACAGTGATGTCATGGCCAGCGACAATGGCGGTGCGGTC
  AAGCTTCCACAGCTGTGCAAGTTTTGCGATGTGAGACTGTCCACTTGCGACAACCAGAAGTCCTGCATGA
  GCAACTGCAGCATCACGGCCATCTGTGAGAAGCCGCATGAAGTCTGCGTGGCCGTGTGGAGGAAGAACGA
  CAAGAACATTACTCTGGAGACGGTTTGCCACGACCCCAAGCTCACCTACCACGGCTTCACTCTGGAAGAT
  GCCGCTTCTCCCAAGTGTGTCATGAAGGAAAAGAAAAGGGCGGGCGAGACTTTCTTCATGTGTGCCTGTA
  ACATGGAAGAGTGCAACGATTACATCATCTTTTCGGAAGAATACACCACCAGCAGTCCCGACCTGTTGTT
  GGTCATTATCCAAGTGACGGGTGTCAGCCTCCTGCCTCCGCTGGGGATTGCCATAGCTGTCATCATCATC
  TTCTACTGCTACCGTGTCCACCGGCAGCAGAAGCTGAGCCCGTCCTGGGAGAGCAGCAAGCCCCGGAAAC
  TGATGGATTTCAGTGACAATTGTGCCATCATCCTGGAGGACGACCGCTCCGACATCAGCTCCACGTGCGC
  CAACAACATCAACCACAACACGGAGCTGCTGCCCATCGAGCTGGACACGCTGGTGGGGAAGGGCCGCTTC
  GCCGAGGTCTACAAGGCCAAGCTGAAGCAGAACACCTCAGAGCAGTTTGAGACCGTGGCTGTCAAGATCT
  TCCCCTACGAGGAGTACTCCTCGTGGAAAACAGAGAAGGACATCTTCTCCGATATCAACCTGAAGCATGA
  GAACATCCTGCAGTTCCTGACGGCCGAGGAGCGGAAGACAGAGCTGGGCAAGCAGTACTGGCTGATCACG
  GCGTTCCACGCGAAGGGCAACCTGCAGGAGTACCTCACGAGGCATGTCATCAGCTGGGAGGACCTGAGGA
  AGCTGGGCAGCTCCCTGGCCCGGGGCATCGCTCATCTCCACAGTGACCACACTCCTTGTGGGAGGCCCAA
  GATGCCCATTGTTCACAGGGACCTCAAGAGCTCTAACATCCTAGTGAAGAACGACTTGACCTGTTGCCTG
  TGTGACTTCGGGCTGTCCTTGCGCCTGGACCCTACTCTGTCTGTGGATGACCTGGCCAACAGCGGGCAGG
  TGGGAACGGCAAGATACATGGCCCCGGAAGTTCTAGAATCCAGGATGAATCTGGAAAACGTGGAGTCGTT
  CAAGCAGACGGATGTCTACTCCATGGCTCTGGTACTCTGGGAAATGACGTCCCGCTGCAATGCTGTGGGA
  GAAGTGAAGGATTACGAGCCCCCATTTGGTTCCAAGGTGCGGGAGCACCCCTGTGTGGAGAGCATGAAAG
  ACAGTGTGCTGAGAGACCGAGGGCGGCCGGAAATTCCCAGCTTCTGGCTCAACCACCAGGGCATCCAGAT
  CGTGTGTGAGACTTTGACCGAGTGCTGGGACCATGACCCCGAAGCCCGTCTCACAGCACAGTGTGTGGCA
  GAGCGCTTCAGTGAGCTGGAGCATCCGGAGAGACTCTCTGGGAGGAGCTGCTCCCAGGAGAAGATTCCAG
  AAGATGGCTCGCTGAACACTACCAAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC052629
• Insert Size: 720 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052629
• RefSeq Size: 8165 bp
• RefSeq ORF: 1778 bp
• Locus ID: 21813
• Cytogenetics: 9 68.39 cM
• Gene Summary: Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways (By similarity).[UniProtKB/Swiss-Prot Function]