Stxbp4 (BC032881) Mouse Untagged Clone

CAT#: MC218250

Stxbp4 (untagged) - Mouse syntaxin binding protein 4 (cDNA clone MGC:41138 IMAGE:1224878), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Stxbp4
• Synonyms: 6030470M02Rik; Synip
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC032881
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGATGGTACAGCTTCTGCCCGATCATCCAGCCCACTTGACAGGGATCCTGCCTTTCGGGTGATTA
  CAGTTACTAAGGAAACAGGTCTAGGTCTGAAGATCCTAGGCGGAATTAACAGGAATGAAGGACCGCTGGT
  GTATATTCACGAAGTCATTCCTGGAGGTGACTGTTACAAGGATGGACGTTTGAAGCCAGGAGATCAACTT
  GTCTCAATAAACAAGGAATCTATGATTGGTGTATCATTTGAAGAAGCAAAAAGCATAATTACCAGAGCCA
  AGTTGAGGTCAGAATCTCCCTGGGAGATAGCATTCATCAGACAAAAGTCTTACTGTGGCCATCCAGGAAA
  TATTTGCTGTCCATCCCCACAAGTGTCAGAAGACTGTGGACCTCAAACCTCAACATTTACTCTTCTTTCC
  TCTCCCTCTGAAACACTACTTCCAAAGACTTCATCCACTCCCCAGACTCAGGACTCCACTTTCCCTTCTT
  GTAAAGCAATTCAGACAAAACCTGAACACGATAAAACAGAACATAGTCCAATTACTTCTTTGGACAACAG
  CCCTGCAGATACATCTAATGCAGACATTGCTCCAGCCTGGACTGATGATGATTCTGGACCACAAGGAAAG
  ATTTCCCTAAATCCTTCTGTTCGCCTTAAGGCAGAGAAACTGGAAATGGCTCTCAATTACCTCGGTATAC
  AGCCAACAAAGGAACAACGTGAAGCCCTGAGAGAGCAAGTCCAGGCCGACTCAAAGGGGACTGTGTCTTT
  TGGAGATTTCGTCCAGGTTGCCAGAAGTTTGTTTTGCTTGCAGTTGGATGAAGTAAATGTTGGTGTCCAT
  GAAATCCCCAGCATCTTAGACTCACAGCTTCTCCCCTGTGATTCTCTAGAAGCAGATGAAGTGGGAAAAC
  TTAGACAAGAAAGAAACGCTGCTCTAGAGGAACGGAATGTGCTTAAGGAGAAGTTACTGGAATCAGAAAA
  GCACAGGAAACAATTGATAGAAGAACTCCAGAATGTGAAGCAGGAAGCCAAAGCTGTAGCTGAGGAAACC
  CGAGCTCTGCGAAGCCGGATTCATCTCGCAGAAGCTGCACAGCGGCAGGCACACGGGATGGAAATGGATT
  ATGAAGAGGTGATCCGTCTGCTAGAGGCTGAGGTCTCAGAACTAAAGGCTCAGCTCGCTGATTATTCTGA
  CCAAAATAAAGAAAGTGTCCAGGACTTGAGAAAAAGAGTCACCGTTCTTGACTGCCAATTGCGAAAATCA
  GAAATGGCTCGGAAAGCATTCAAGGCGTCCACTGAAAGGCTCCTTGGTTTCATAGAGGCTATTCAAGAAG
  TTCTTTTGGATAGTTCTGCTCCTTTATCAACTTTAAGTGAAAGAAGAGCTGTGCTCGCATCTCAGACTTC
  GCTTCCACTGCTGGCAAGGAACGGACGCAGCTTCCCAGCAACCCTGCTTCTGGAATCCAAGGAGCTTGTC
  AGATCTGTCCGTGCCATACTTGATATGGACTGTAAGTTATCATTATCATCTTCTTCTTCTCCTTCCTCCT
  CCTCCTCCTTCTCCTCCTCTTCTTCTTGTAATTTATTAAACAAAGCAGAAGAAAGACCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC032881
• Insert Size: 1602 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC032881, AAH32881
• RefSeq Size: 1807 bp
• RefSeq ORF: 1601 bp
• Locus ID: 20913
• Cytogenetics: 11 D
• Gene Summary: Plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane. Inhibits the translocation of SLC2A4 from intracellular vesicles to the plasma membrane by STX4A binding and preventing the interaction between STX4A and VAMP2. Stimulation with insulin disrupts the interaction with STX4A, leading to increased levels of SLC2A4 at the plasma membrane. May also play a role in the regulation of insulin release by pancreatic beta cells after stimulation by glucose.[UniProtKB/Swiss-Prot Function]