Snap91 (BC052454) Mouse Untagged Clone

CAT#: MC218238

Snap91 (untagged) - Mouse synaptosomal-associated protein 91 (cDNA clone MGC:63436 IMAGE:6410678), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Snap91
• Synonyms: AP180, F1-20, 91kDa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052454
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCCTTGAACAACATCTAAATACCCTAGAAGGAAAGAAACCTGGAAACAATGAAGGATCTGGTG
  CTCCCTCTCCACTAAGTAAGTCTTCTCCAGCCACAACTGTTACATCTCCTAATTCTACACCAGCTAAAAC
  TATCGACACATCCCCGCCAGTTGACATATTTGCAACAGCATCCGCGGCTGCCCCAGTCAGCTCTGCTAAG
  CCATCAAGCGATCTCCTTGATCTTCAGCCCGACTTCTCTGGAGCAGCTGCGGGGGCAGCAGCACCTGTAG
  TGCCTCCTTCTGGGGGTGCGACCGCCTGGGGAGACCTTTTGGGAGAGGATTCCTTGGCTGCACTTTCCTC
  TGTTCCCTGTGAAGCACCGATTTCAGACCCATTTGCACCAGAGCCTTCCCCTCCTACTACAACCACTGAG
  CCTGCTTCAGCCTCTGCCTCGACCACCACAGCTGTGACGGCTGTCACTACGGAAGTGGATCTCTTTGGAG
  ATGCCTTTGCAGCTTCTCCTGGGGAGGCCCCTGCAGCATCCGAAGGGGCTACCGCACCAGCTACCCCGGC
  CCCAGTGGCTGCAGCTCTTGATGCATGCTCAGGAAATGACCCTTTTGCCCCATCTGAAGGTAGCGCAGAG
  GCTGCACCTGAGCTGGACCTCTTTGCAATGAAGCCACCTGAGACCAGCGCTCCTGTAGTTACCCCTACAG
  CTAGCACAGCCCCTCCAGTTCCCGCAACTGCTCCTTCTCCTGCTCCCACGGCTGTGGCAGCCACTGCTGC
  CACCACCACCGCCGCCGCTGCAGCTACCACCACTGCCACCACCTCTGCTGCTGCTGCCACCACCGCCGCT
  GCTCCTCCTGCTCTAGATATCTTTGGTGATTTGTTTGATTCTGCTCCTGAAGTTGCTGCAGCACCTAAGC
  CAGACGCGGCTCCTAGCATAGACCTGTTTGGCACAGATGCTTTCTCCTCCCCGCCACGAGGGGCCTCTCC
  GGTGCCTGAGAGTTCTCTCACTGCTGACCTCTTATCTGTGGACGCATTTGCAGCGCCGTCTCCTGCATCC
  ACTGCCTCTCCTGCAAAGGCGGAGTCCTCGGGTGTCATAGACCTTTTTGGGGATGCGTTTGGAAGTGGTG
  CTTCTGAAACCCAGCCAGCACCACAGGCTGTTTCTAGTTCATCAGCATCGGCAGATCTACTAGCTGGATT
  TGGGGGTTCTTTCATGGCCCCTTCTACAACGCCAGTGACTCCAGCTCAGAATAACCTGCTGCAACCCAGT
  TTCGAGGCAGCTTTTGGAACGACGCCTTCGACTTCAAGCAGCAGCTCTTTTGACCCATCAGTGTTTGATG
  GTTTAGGCGATCTTCTGATGCCAACCATGGCACCATCCGGGCAGCCTGCCCCTGTCTCAATGGTCCCACC
  CAGTCCTGCAATGGCAGCCAGCAAAGGCCTCGGAAGTGACCTTGACTCGTCTCTGGCCAGTTTAGTAGGC
  AATCTTGGGATTTCTGGTACCACATCAAAAAAGGGAGATCTCCAGTGGAATGCTGGGGAGAAAAAGCTGA
  CTGGTGGAGCCAACTGGCAACCGAAAGTCACTCCAGCCACATGGTCAGCGGGTGTTCCCCCGCAAGGCAC
  TGTTCCACCAACCAGCTCAGTCCCTCCGGGTGCCGGGGCCCCGTCCGTTGGGCAACCTGGAGCAGGATTT
  GGAATGCCTCCTTCAGGGACAGGCATGACCATGATGTCTCAGCAGCCAGTCATGTTTGCACAGCCCATGA
  TGAGGCCACCCTTTGGAGCTGCAGCTGTGCCCGGCACACAGCTTTCTCCAAGCCCTACACCTGCCACTCA
  GAGTCCCAAGAAACCTCCAGCCAAGGACCCGTTAGCGGATCTTAACATCAAGGATTTCTTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC052454
• Insert Size: 1884 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052454, AAH52454
• RefSeq Size: 4519 bp
• RefSeq ORF: 1883 bp
• Locus ID: 20616
• Cytogenetics: 9 E3.1
• Gene Summary: Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Binding of AP180 to clathrin triskelia induces their assembly into 60-70 nm coats.[UniProtKB/Swiss-Prot Function]