Pklr (NM_001099779) Mouse Untagged Clone

CAT#: MC218222

Pklr (untagged) - Mouse pyruvate kinase liver and red blood cell (Pklr), nuclear gene encoding mitochondrial protein, transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pklr
• Synonyms: Pk-1; Pk1; R-PK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218222 representing NM_001099779
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGGCCAGCAGGATACCTGAGACGTGCCAGTGTGGCCCAGCTGACCCAGGAGCTGGGCACTGCCT
  TCTTCCAGCAGCAGCAACTGCCCGCAGCTATGGCGGACACCTTCCTGGAACACCTCTGCCTTCTGGATAT
  CGACTCAGAGCCTGTGGCCGCTAGGAGCACCAGCATCATTGCCACCATCGGGCCGGCGTCACGCTCTGTG
  GACCGCCTCAAGGAGATGATCAAGGCAGGGATGAACATTGCACGACTCAACTTCTCCCATGGCTCCCATG
  AGTACCATGCAGAGTCCATCGCCAACATTCGGGAGGCGGCTGAAAGCTTTGCAACCTCCCCACTCAGCTA
  CAGACCCGTGGCCATCGCCCTGGACACCAAGGGTCCCGAGATACGCACTGGAGTCCTGCAGGGGGGTCCA
  GAGTCGGAGGTGGAAATTGTGAAGGGTTCACAGGTGCTGGTGACTGTGGATCCGAAGTTCCGGACAAGGG
  GCGATGCAAAGACAGTGTGGGTGGACTACCACAATATCACCCAGGTCGTTGCAGTGGGGGGCCGCATCTA
  CATTGACGACGGGCTCATCTCCTTAGTGGTGCGGAAAATTGGCCCAGAGGGACTGGTGACCGAAGTGGAA
  CACGGTGGTTTCTTGGGCAACAGGAAGGGTGTGAACTTGCCAAATGCCGAGGTGGACCTGCCTGGGCTAT
  CAGAGCAAGATCTTTTGGATCTGCGCTTCGGGGTGGAGCATAATGTGGACATCATCTTTGCCTCCTTTGT
  ACGAAAAGCCAGTGATGTGGTGGCAGTCCGAGATGCCCTAGGGCCAGAAGGACGGGGCATCAAAATTATC
  AGCAAAATCGAGAACCATGAAGGCGTGAAGAAGTTTGATGAGATCCTAGAAGTGAGCGATGGCATCATGG
  TGGCTCGGGGTGACCTTGGCATTGAGATCCCAGCAGAGAAGGTTTTCTTGGCTCAGAAGATGATGATTGG
  ACGCTGCAACCTGGCTGGCAAGCCTGTCGTTTGTGCCACACAGATGCTGGAGAGCATGATCACTAAGGCT
  CGACCAACTCGGGCGGAGACAAGCGATGTGGCCAATGCTGTGCTGGATGGGGCTGACTGTATCATGCTGT
  CTGGAGAGACCGCCAAAGGCAGTTTCCCCGTGGAGGCTGTAAAGATGCAACATGCGATTGCCCGGGAGGC
  AGAGGCCGCTGTGTACCACCGCCAGTTGTTTGAGGAGCTACGCCGGGCAGCGCCGCTGAGCCGTGACCCA
  ACCGAGGTCACTGCAATTGGAGCCGTGGAGGCTTCCTTCAAGTGCTGTGCCGCAGCCATCATTGTGCTGA
  CAAAGACTGGCCGCTCAGCTCAGCTTCTGTCTCGCTACCGACCTCGGGCTGCTGTCATTGCTGTGACTCG
  TTCTGCCCAGGCTGCCCGACAGGTCCACCTGTCCCGAGGAGTCTTCCCCTTGCTCTACCGTGAGCCTCCA
  GAGGCTGTCTGGGCAGATGATGTGGACCGAAGGGTCCAATTTGGCATCGAAAGTGGAAAGCTTCGTGGCT
  TCCTCCGAGTTGGTGATCTGGTGATTGTGGTGACAGGCTGGCGACCTGGCTCTGGCTATACCAACATTAT
  GCGGGTGCTGACCATATCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001099779
• Insert Size: 1632 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001099779.1, NP_001093249.1
• RefSeq Size: 2661 bp
• RefSeq ORF: 1632 bp
• Locus ID: 18770
• Cytogenetics: 3 39.01 cM