Pgk1 (BC083355) Mouse Untagged Clone

CAT#: MC218218

Pgk1 (untagged) - Mouse phosphoglycerate kinase 1 (cDNA clone MGC:102158 IMAGE:6828087), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pgk1
• Synonyms: Pgk-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC083355
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCTTTCCAACAAGCTGACTTTGGACAAGCTGGACGTGAAGGGGAAGCGGGTCGTGATGAGGGTGG
  ACTTCAACGTTCCTATGAAGAACAACCAGATAACAAACAACCAAAGGATCAAGGCTGCTGTTCCAAGCAT
  CAAATTCTGCTTGGACAATGGAGCCAAGTCCGTTGTCCTTATGAGCCACCTGGGCCGGCCTGATGGTGTT
  CCCATGCCTGACAAGTACTCCTTAGAGCCAGTTGCTGCTGAACTCAAATCTCTGCTGGGCAAGGATGTTC
  TGTTCTTGAAGGATTGTGTGGGCCCAGAAGTCGAGAATGCCTGTGCCAACCCAGCGGCTGGGACTGTCAT
  CCTGCTGGAAAACCTCCGCTTTCATGTAGAGGAAGAAGGGAAGGGAAAAGATGCTTCTGGAAACAAGGTT
  AAAGCTGAGCCGGCCAAAATTGATGCTTTCCGAGCCTCACTGTCCAAACTAGGAGATGTCTATGTCAATG
  ATGCTTTTGGGACTGCACACCGAGCCCATAGCTCCATGGTGGGTGTGAATCTGCCACAGAAGGCTGGTGG
  ATTTTTGATGAAGAAGGAGCTGAACTACTTTGCCAAGGCTTTGGAGAGTCCAGAGCGACCCTTCCTGGCT
  ATCTTGGGAGGCGCTAAAGTTGCAGACAAGATCCAGCTGATCAATAATATGCTAGACAAAGTCAATGAGA
  TGATCATTGGTGGTGGAATGGCCTTTACCTTCCTTAAGGTGCTCAACAACATGGAGATTGGCACATCTCT
  GTATGATGAAGAAGGAGCCAAGATTGTCAAAGATCTCATGTCCAAAGCTGAGAAAAATGGTGTGAAGATT
  ACCTTGCCTGTTGACTTTGTCACTGCTGACAAATTTGATGAGAATGCCAAGACTGGCCAAGCTACTGTGG
  CCTCTGGTATACCTGCTGGCTGGATGGGCTTGGACTGTGGTACTGAGAGCAGCAAGAAATATGCCGAGGC
  TGTGGGTCGAGCTAAGCAGATTGTTTGGAATGGTCCTGTTGGGGTATTTGAATGGGAAGCCTTTGCCAGG
  GGAACCAAGTCACTCATGGATGAGGTGGTGAAAGCCACTTCTAGGGGTTGCATCACTATCATAGGTGGTG
  GAGACACTGCCACTTGCTGTGCCAAATGGAACACAGAGGATAAAGTCAGCCATGTGAGCACTGGGGGCGG
  TGCCAGTCTAGAGCTCCTGGAAGGTAAAGTCCTTCCTGGGGTGGATGCTCTCAGCAATGTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC083355
• Insert Size: 1254 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC083355, AAH83355
• RefSeq Size: 1727 bp
• RefSeq ORF: 1253 bp
• Locus ID: 18655
• Cytogenetics: X 47.36 cM
• Gene Summary: The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Many pseudogenes of this gene are found throughout the mouse genome. [provided by RefSeq, Jan 2014]