Pak1 (NM_011035) Mouse Untagged Clone

CAT#: MC218214

Pak1 (untagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 1 (Pak1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pak1
• Synonyms: AW045634; PAK-1; Paka
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218214 representing NM_011035
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAATAACGGCGTAGACATCCAGGACAAACCCCCAGCCCCTCCGATGAGAAACACCAGCACTATGA
  TTGGAGCCGGCAGCAAAGACACTGGAACCCTAAACCACGGCTCCAAACCTCTGCCTCCAAACCCAGAGGA
  GAAGAAAAAGAAGGACCGGTTTTATCGATCCATCTTACCTGGAGATAAAACAAATAAAAAGAAGGAGAAG
  GAGCGACCAGAGATTTCTCTTCCTTCAGATTTTGAGCATACAATTCATGTTGGTTTTGATGCTGTCACAG
  GGGAGTTTACGGGAATGCCAGAACAGTGGGCTCGCTTGCTTCAAACATCAAATATCACAAAGTCAGAACA
  GAAGAAAAACCCACAGGCTGTTCTGGATGTGTTGGAATTTTATAACTCTAAGAAGACCTCCAATAGTCAG
  AAGTACATGAGTTTTACAGATAAGTCAGCTGAAGATTATAATTCTTCTAACACTTTGAATGTGAAGACTG
  TGTCTGAGACCCCAGCAGTACCACCAGTGTCAGAAGATGAAGATGATGATGACGATGCTACCCCACCTCC
  AGTGATTGCTCCACGCCCAGAACACACAAAATCTGTATATACACGATCTGTGATTGAACCACTTCCTGTT
  ACTCCAACTCGGGATGTGGCTACATCTCCTATTTCTCCTACTGAGAATAACACCACTCCGCCAGATGCTT
  TGACCCGGAACACGGAAAAACAGAAGAAGAAGCCTAAAATGTCTGATGAGGAGATCTTAGAGAAATTACG
  GAGCATAGTGAGTGTGGGTGATCCTAAGAAGAAGTACACACGGTTCGAGAAGATTGGACAAGGTGCTTCA
  GGCACAGTGTATACTGCAATGGATGTAGCCACAGGGCAGGAGGTGGCCATTAAACAGATGAATCTTCAGC
  AGCAGCCGAAGAAAGAGCTGATTATTAATGAGATCCTGGTCATGAGGGAAAACAAAAACCCAAATATTGT
  CAACTACCTGGACAGTTACCTTGTGGGAGATGAGCTGTGGGTTGTTATGGAATACTTGGCTGGAGGCTCC
  TTGACAGATGTGGTGACAGAAACCTGTATGGATGAAGGCCAGATAGCAGCTGTGTGCCGAGAGTGTCTAC
  AAGCTTTGGAGTTTCTACATTCAAACCAAGTCATTCACAGGGACATCAAGAGTGACAATATTCTGCTGGG
  AATGGATGGCTCTGTCAAGTTAACTGACTTTGGATTCTGTGCACAGATAACTCCAGAGCAGAGCAAAAGG
  AGCACCATGGTGGGAACTCCATATTGGATGGCACCTGAAGTTGTGACACGCAAGGCCTATGGACCCAAGG
  TTGACATCTGGTCCCTGGGCATTATGGCAATTGAAATGATTGAGGGGGAGCCCCCATACCTCAATGAAAA
  CCCTTTGAGAGCCTTGTACCTCATTGCTACCAATGGGACGCCAGAGCTTCAGAACCCAGAGAAGTTGTCA
  GCTATTTTCCGGGACTTCCTGAACCGCTGTCTTGAGATGGATGTGGAGAAGAGAGGCTCAGCTAAAGAGC
  TGCTGCAGCATCAGTTCCTGAAGATTGCCAAGCCCCTCTCTAGCCTGACTCCACTGATTGCTGCAGCAAA
  AGAGGCAACCAAGAACAATCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011035
• Insert Size: 1635 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011035.2, NP_035165.2
• RefSeq Size: 3081 bp
• RefSeq ORF: 1635 bp
• Locus ID: 18479
• Cytogenetics: 7 53.57 cM