Oc90 (BC048716) Mouse Untagged Clone

CAT#: MC218212

Oc90 (untagged) - Mouse otoconin 90 (cDNA clone MGC:59513 IMAGE:6334417), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Oc90
• Synonyms: PLA2L, Ocn-95
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC048716
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTATGCTGCTCATGGTCGGTATGCTGATGGCCCCCTGTGTTGGGGCCCATGCTCTGGACACACCAA
  ATCCCCAGGAATTGCCTCCAGGACTGTCAAAAAATATAAATATCACTTTCTTCAATGGAGTGTTTAAAAA
  CGTGGAAAGTGTGGCTGAAATTTTTGACTGCCTAGGTTCCCACTTCACCTGGCTGCAGGCTGTCTTCACC
  AACTTCCCGCTGCTCCTCCAGTTTGTAAACAGTATGAGGTGTGTAACTGGCCTCTGCCCCCGGGACTTTG
  AAGACTATGGTTGTGCCTGTAGGTTTGAGATGGAAGGGATGCCTGTGGACGAGTCTGATATCTGCTGCTT
  CCAGCATCGCAGGTGCTATGAGGAAGCTGTTGAGATGGACTGTCTCCAAGACCCTGCCAAGCTCAGTGCA
  GATGTGGATTGCACCAACAAACAGATCACATGTGAGTCCGAGGATCCCTGTGAGCGTCTACTGTGTACGT
  GTGACAAGGCTGCTGTGGAGTGCCTGGCTCAGTCTGGCATCAACTCCTCCCTGAACTTCCTGGATGCTTC
  CTTCTGCCTCCCTCAGACTCCAGAGACAACTAGTGGGAAGGCTGCAACACTGTTGCCTAGAGAGTCTGTT
  CAAGATCTTCAAGACACACAAGCTTCTAGGACCACATCAAGTCCAGGATCTGCAGAGATTATTGCCCTAG
  CCAAAGGTACAACCCACTCTGCTGGCATCAAACCACTGAGGTTGGGAGTCTCATCTGTTGACAATGGTTC
  CCAGGAAGCAGCTGGAAAAGCAGCCTGTGACAGATTGGCCTTCGTGCATCTGGGTGATGGGGACAGCATG
  ACGGCCATGCTGCAGCTTGGAGAGATGCTCTTCTGTCTAACATCCCATTGCCCAGAGGAATTTGAAACTT
  ACGGCTGCTACTGTGGAAGAGAAGGAAGAGGAGAGCCAAGGGACACCCTGGATAGGTGCTGTCTGTCCCA
  TCACTGCTGTTTGGAGCAGATGAGACAAGTGGGCTGCCTCCATGGAAGGCGTTCTCAGTCATCTGTGGTA
  TGTGAAGACCACATGGCCAAATGTGTGGGGCAGAGCCTGTGTGAGAAGCTACTATGTGCCTGTGACCAGA
  TGGCAGCTGAGTGCATGGCCTCTGCCTTCTTTAATCAAAGCCTCAAGTCACCAGACGGAGCCGAGTGTCA
  AGGCGAGCCTGTGTCCTGTGAGGATGGCATGCTCCAGGGCACCTTGGCCTCTTCTGTGGACTCCAGTTCT
  GAGGAGAATAGTGAGGAAGCTCCACCGCAGATGGAACGCCTAAGAAGATTTCTGGAAAAGCCTCCTGGTC
  CCTTGGGGGCCAGACCCCTCGGTGGGAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC048716
• Insert Size: 1362 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC048716, AAH48716
• RefSeq Size: 1701 bp
• RefSeq ORF: 1361 bp
• Locus ID: 18256
• Cytogenetics: 15 29.16 cM
• Gene Summary: Major protein of the otoconia, a calcium carbonate structure in the saccule and utricle of the ear (PubMed:17300776). Together with OTOL1, acts as a scaffold for otoconia biomineralization: sequesters calcium and forms interconnecting fibrils between otoconia that are incorporated into the calcium crystal structure (PubMed:21655225, PubMed:24748133). Together with OTOL1, modulates calcite crystal morphology and growth kinetics (PubMed:24748133). It is unlikely that this protein has phospholipase A2 activity (PubMed:17300776).[UniProtKB/Swiss-Prot Function]