Krt13 (BC060286) Mouse Untagged Clone

CAT#: MC218195

Krt13 (untagged) - Mouse keratin 13 (cDNA clone MGC:70026 IMAGE:30292772), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Krt13
• Synonyms: CK13, K13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC060286
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTGCCGCTTCCAGAGTTCCTCCATGAGCTATGGAGGTGGTTTCGGAGCTGGTTCTTGCCAGCTTG
  GAGGAGGCCGTAATATCTCCTCCTGCTCATCTCGGTTTGTCACTGGAGGGTCAGCTGGAGGCTATGGAGG
  GGGCATGAGCTGTGGCTTTGGTGGAGGAGCTGGTGGTGGCTTTGGAGGTGGCTTTGGAGGTGGCTTTGGA
  GGTAGCTATGGCGGAGGTTTTGGCGGTGGCTTTGGTGACTTCGGAGGTGTCGATGGTGGCCTCCTCTCTG
  GCAACGAGAAGATCACCATGCAGAACCTCAATGACCGCCTGGCCAGCTACCTGGATAAGGTGCGCGCCTT
  GGAGGCAGCCAATGCCGACCTGGAGGTGAAGATTCGTGACTGGCATCTGAAACAGAGCCCAGCTAGTCCA
  GAGCGGGACTACAGTGCTTATTATAAGACCATCGAGGAGCTCCGGATCAAGATTCTGGAAGCCACCACGG
  ACAACAACCGGATCATCCTGGAGATTGACAATGCCAGGCTGGCTGCAGATGACTTCAGGCTCAAGTACGA
  AAATGAGCTGACCTTGCGCCAGAGCGTGGAGGCCGACATCAACGGCCTGCGCAGGGTGCTGGATGAGCTG
  ACTCTGGCTAAGACTGACCTGGAAATGCAGATTGAGAGCCTGAATGAAGAACTGGCTTACCTGAAGAAGA
  ACCACGAAGAGGAGATGAAGGAATTCAGCAACCAGGTAGTAGGCCAGGTCAATGTGGAGATGGACGCCAC
  CCCTGGCATTGATCTGACCCGTGTGCTGGCTGAGATGAGGGAGCAGTATGAAGCCCTGGCAGAGAAGAAT
  CGGAGGGATGCTGAGGAATGGTTCCAGACCAAGAGTGCAGAGCTGAACAAGGAAGTATCCTCCAATTCCG
  GTCGCCCAGATTTCCGAAAGTATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC060286
• Insert Size: 936 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC060286, AAH60286
• RefSeq Size: 1232 bp
• RefSeq ORF: 935 bp
• Locus ID: 16663
• Cytogenetics: 11 63.41 cM
• Gene Summary: The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]