Gnrhr (BC061250) Mouse Untagged Clone

CAT#: MC218173

Gnrhr (untagged) - Mouse gonadotropin releasing hormone receptor (cDNA clone MGC:74406 IMAGE:30249439), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gnrhr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC061250
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAACAATGCATCTCTTGAGCAGGACCCAAATCACTGCTCGGCCATCAACAACAGCATCCCCTTGA
  TACAGGGCAAGCTCCCGACTCTAACCGTATCTGGAAAGATCCGAGTGACCGTGACTTTCTTCCTTTTCCT
  ACTCTCTACTGCCTTCAATGCTTCCTTCTTGTTGAAGCTGCAGAAGTGGACTCAGAAGAGGAAGAAAGGA
  AAAAAGCTCTCAAGGATGAAGGTGCTTTTAAAGCATTTGACCTTAGCCAACCTGCTGGAGACTCTGATCG
  TCATGCCACTGGATGGGATGTGGAATATTACTGTTCAGTGGTATGCTGGGGAGTTCCTCTGCAAAGTTCT
  CAGCTATCTGAAGCTCTTCTCTATGTATGCCCCAGCTTTCATGATGGTGGTGATTAGCCTGGACCGCTCC
  CTGGCCATCACTCAGCCCCTTGCTGTACAAAGCAACAGCAAGCTTGAACAGTCTATGATCAGCCTGGCCT
  GGATTCTCAGCATTGTCTTTGCAGGACCACAGTTATATATCTTCAGGATGATCTACCTAGCAGACGGCTC
  TGGGCCCACAGTCTTCTCGCAATGTGTGACCCACTGCAGCTTTCCACAGTGGTGGCATCAGGCCTTCTAC
  AACTTTTTCACCTTCGGCTGCCTCTTCATCATCCCCCTCCTCATCATGCTAATCTGCAATGCCAAAATCA
  TCTTTGCTCTCACGCGAGTCCTTCATCAAGACCCACGCAGTACGGTATTCCTTAGATCTAAGAATCACTG
  TAGACAAACGGAATTTAAACAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC061250
• Insert Size: 795 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC061250, AAH61250
• RefSeq Size: 1500 bp
• RefSeq ORF: 794 bp
• Locus ID: 14715
• Cytogenetics: 5 43.56 cM
• Gene Summary: Receptor for gonadotropin releasing hormone (GnRH) that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle-stimulating hormone (FSH). This receptor mediates its action by association with G-proteins that activate a phosphatidylinositol-calcium second messenger system.[UniProtKB/Swiss-Prot Function]