Evx2 (BC061467) Mouse Untagged Clone

CAT#: MC218166

Evx2 (untagged) - Mouse even skipped homeotic gene 2 homolog (cDNA clone MGC:67086 IMAGE:6827284), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Evx2
• Synonyms: Evx-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC061467
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAAAGAATAAGAAAAGAGATGATTCTGATGGAAAGAGGGCTCCACAGCCCTACTGCTGGCAAGA
  GGTTCTCCAGTTTGTCCGATTCAGCTGGCGGTGCTGTGCTCGAGGCCCTGGAAAATTCGCAGCACCCGGC
  TCGCCTCAGTCCGCGCCTGCCGTCCGCCCCGCTGCACGGAGCTCTGGGAGACCTCCCCGCCAAAGGCAAA
  TTCGAAATAGACACTTTGTTCAACCTGCAGCACCCGAGCAGCGAAAGCACCGTCTCCTCCGAAATTGCCT
  CCGCTACCGAGAGCCGCAAGAAGCCTAGTCATTATTCCGAAGCGGCCGCCGAGGCCGACATGAGCAGCGA
  CGTGGAGGTGGGCTGCTCCGCACTGCGCTCCCCCAGCGGCCTGGGCGCTGCACCGCTCAAGGAAAACAAT
  GCCAAAGGGTACACGGAGAGCGGCTCCGTCGCGGGCACCACGACGTCGGCTTCCGGCTCGGGCCTCGGTA
  GTCTGCATGGAGGCGGCGGCGGCGGCAATAGCGGGGCTGCGGCGTTGGGCGGCTCTGGCTCCGGTTCCGG
  CGCCGATCAGGTGCGGCGATACCGTACGGCATTCACCCGCGAACAGATCGCGCGCCTGGAGAAAGAGTTC
  TACAGGGAGAACTACGTGTCTCGGCCCCGCCGGTGCGAGCTGGCCGCTGCACTCAACCTGCCCGAAACCA
  CCATCAAGGTGTGGTTCCAGAACCGGCGCATGAAGGACAAACGGCAGCGCCTGGCCATGTCGTGGCCGCA
  TCCAGCCGACCCCAGCTTCTACACCTACATGATGACACACGCGGCGGCCACCGGAAGCCTACCCTACCCT
  TTTCACTCGCACGTGCCGCTGCACTACTACCCGCACGTGGGCGTCACCGCGGCTGCAGCAGCGGCCGCAG
  CCTCGGGAGCGGCGGCGGCAGCATCCTCGCCCTTCGCCACATCCATCCGTCCTCTGGACACCTTCCGTGC
  GCTTTCACATCCTTATTCGCGGCTGGGGCGCCCCCCAGCGGTAGCTCGGCGCCCTGCTCGTGCCTCAGTT
  GTCACAGCAGCCAGTCTGCAGCGGCGGCGGCCGCCGCTGCAGCGGCAGCGCTGGGCTCCCGGGGCGGCGG
  TGGCAGCGGCGGTGGCGGCGGCGGGGGAGCCGGGACGGCGGGTGGCTCGGACTTTGGCTGCAGCGCCGCG
  GCGCCGCGCTCCGAGAGCGGCTTCTTGCCCTATTCCGCCGCGGTGCTCAGCAAGACCGCCGTCAGCCCGC
  CGGACCAGAGGGACGAGGCGCCGCTCACCAGATAACTACGCCGCCGCCAGGGGCCGCGCCAGCCTCTCGG
  CGTGCGCCCCGTCGGGCCCCGCTTGGCCCTCGGCTCCTGCTGCCGCTAGGGGGCGCCCCGGGGGGTCCGG
  GAGGGCGAAAGAAACCTGCCCGAGTTGGGGGGTTGGGGGAGGAGGGAAGGGGCGGGCGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC061467
• Insert Size: 1461 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC061467, AAH61467
• RefSeq Size: 4077 bp
• RefSeq ORF: 1460 bp
• Locus ID: 14029
• Cytogenetics: 2 44.13 cM
• Gene Summary: This gene is located at the 5' end of the Hoxd gene cluster on chromosome 2. The encoded protein is a homeobox transcription factor that is related to the protein encoded by the Drosophila even-skipped (eve) gene, a member of the pair-rule class of segmentation genes. The encoded protein plays a role in limb morphogenesis. [provided by RefSeq, Aug 2015]