Grb10 (NM_001177629) Mouse Untagged Clone

CAT#: MC218095

Grb10 (untagged) - Mouse growth factor receptor bound protein 10 (Grb10), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Grb10
• Synonyms: 5730571D09Rik; AI325020; Meg1; mKIAA0207
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218095 representing NM_001177629
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAACGATATTAACTCGTCCGTGGAAAGCCTTAACTCAGCTTGCAACATGCAGTCTGATACTGATA
  CTGCACCACTTCTTGAGGATGGCCAGCATGCCAGCAACCAGGGAGCAGCATCTAGCTCCCGGGGACAGCC
  ACAGGCGTCCCCGAGGCAGAAAATGCAACGCTCGCAGCCTGTGCACATTCTCAGGCGCCTTCAGGAGGAA
  GACCAGCAGTTAAGAACTGCATCTCTTCCGGCCATCCCCAACCCATTTCCGGAGCTCACTGGTGCGGCCC
  CTGGGAGCCCTCCTTCGGTTGCTCCTAGCTCCTTACCTCCTCCTCCGAGCCAGCCACCTGCCAAGCATGA
  TGTCAAAGTCTTTAGTGAAGATGGGACCAGCAAAGTGGTGGAGATTCTAACCGACATGACAGCCAGGGAC
  CTGTGCCAGCTGCTGGTTTACAAAAGTCACTGTGTGGATGACAACAGCTGGACTCTGGTGGAACACCACC
  CACAACTGGGATTAGAGAGGTGCCTGGAGGACCATGAGATCGTGGTCCAAGTGGAGAGTACCATGCCAAG
  TGAGAGCAAATTCTTATTCAGAAAGAATTATGCGAAGTACGAGTTCTTTAAGAATCCAGTGAACTTCTTC
  CCGGATCAGATGGTCAATTGGTGCCAGCAGTCCAACGGTGGCCAGGCGCAGCTTCTGCAGAATTTTCTGA
  ACACCAGCAGCTGCCCTGAGATCCAGGGGTTCTTGCAGGTGAAAGAGGTAGGACGCAAGTCTTGGAAGAA
  GCTGTATGTGTGCCTGCGCAGATCTGGCCTCTATTACTCCACCAAGGGGACTTCAAAAGAACCCAGACAC
  CTGCAGCTGCTGGCTGACCTGGAAGAAAGCAGCATCTTCTACCTGATTGCTGGAAAGAAGCAGTACAACG
  CGCCGAATGAACATGGGATGTGCATCAAGCCAAACAAAGCGAAGACCGAGATGAAGGAGCTTCGTCTGCT
  CTGTGCCGAAGATGAGCAGATCCGTACTTGCTGGATGACTGCCTTCAGACTGCTCAAGTACGGAATGCTC
  CTGTACCAAAACTATCGCATCCCACAGAGGAAGGGTCTGCCCCCTCCTTTCAACGCACCTATGCGCAGTG
  TTTCTGAGAATTCTCTTGTGGCCATGGATTTTTCTGGACAAATCGGAAGAGTGATCGATAACCCGGCTGA
  AGCCCAGAGTGCTGCCCTGGAAGAGGGCCATGCCTGGCGTAAGCGGAGCACACGGATGAATATCCTAAGC
  AGCCAAAGCCCACTGCATCCTTCTACCCTGAATGCAGTGATTCACAGGACTCAGCATTGGTTCCATGGAC
  GTATCTCCCGCGAGGAGTCTCACAGGATCATCAAGCAACAAGGTCTCGTGGACGGGCTGTTCCTCCTTCG
  TGACAGCCAGAGTAATCCAAAGGCGTTCGTACTGACACTGTGCCATCACCAGAAGATTAAAAACTTCCAG
  ATCTTACCTTGCGAGGATGATGGGCAGACCTTCTTCACTCTGGATGATGGGAACACCAAGTTCTCCGATC
  TGATCCAGCTGGTCGACTTCTACCAGCTCAACAAAGGTGTTCTGCCCTGCAAGCTGAAACACCACTGCAT
  CCGCGTGGCCTTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001177629
• Insert Size: 1626 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177629.1, NP_001171100.1
• RefSeq Size: 4764 bp
• RefSeq ORF: 1626 bp
• Locus ID: 14783
• UniProt ID: Q60760
• Cytogenetics: 11 7.15 cM
• Gene Summary: Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. A similar role in the mediation of ubiquitination has also been suggested with INSR. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate 5' non-coding exon, and is missing in-frame coding exons compared to variant 3. This results in a shorter isoform (2) lacking an internal protein segment compared to isoform 3.