Sp8 (BC082582) Mouse Untagged Clone

CAT#: MC218053

Sp8 (untagged) - Mouse trans-acting transcription factor 8 (cDNA clone MGC:105193 IMAGE:30653287), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sp8
• Synonyms: D930049B17Rik; mBtd
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC082582
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAACTTCACTTCTAGGGGAAGAACCGAGGTTGGGATCCACTCCTCTGGCCATGCTTGCTGCTACCT
  GTAATAAGATCGGCAGCCCCAGCCCGTCTCCCTCCTCCCTGTCGGACAGCTCTTCTTCCTTCGGTAAAGG
  CTTCCACCCTTGGAAACGCTCCTCGTCTTCCTCCTCCGGCAGCTGCAACGTAGTGGGTTCCAGTCTCTCT
  AGCTTCGGTGTGTCGGGGGCCTCCCGGAACGGCGGCTCCTCCTCAGCCGCAGCGGCGGCAGCGGCGGCAG
  CAGCGGCGGCCGCAGCCCTGGTGTCCGACTCTTTTAGCTGCGGAGGCTCGCCCGGCTCCAGCGCCTTCTC
  TCTCACCTCCAGCAGCGCCGCCGCAGCGGCAGCAGCTGCGGCGGCTGCCGCTTCCAGCTCGCCCTTTGCC
  AACGACTACTCTGTGTTCCAGGCTCCGGGCGTCTCGGGGGGCAGCGGCGGCGGCGGCGGGGGAGGCGGCG
  GCGGTTCCGGAGCGCACTCGCAGGATAGCTCCCATCAGCCAGTGTTTATCTCCAAGGTGCACACGTCGGT
  GGACGGACTGCAGGGTATCTACCCGCGCGTGGGAATGGCGCACCCGTATGAGTCCTGGTTCAAGCCTTCG
  CACCCGGGCCTTGGCGCCGCGGCCGACGTGGGCTCCGCGGGCGCCTCCAGCTGGTGGGATGTGGGCGCGG
  GCTGGATCGACGTGCAGAACCCCAATGGGGCGGCAGCGCTGCCCGGCTCGCTGCACCCGGCGGCCGGGGG
  CCTTCAGACGTCGCTGCATTCACCGCTGGGAGGCTACAACTCGGATTATTCCGGCCTGAGCCACTCCGCT
  CGCCGCTACTCCGGCCGCGCCACTTGCGACTGCCCCAACTGCCAGGAGGCTGAGCGGCTGGGCCCAGCGG
  GAGCGAGCCTGCGTCGCAAAGGGCTGCATAGCTGCCACATCCCGGGCTGCGGCAAGGTGTACGGCAAGAC
  GTCGCACCTGAAGGCGCACCTGCGTTGGCACACCGGCGAGCGGCCGTTCGTGTGCAATTGGCTGTTCTGC
  GGTAAGCGCTTTACGCGCTCCGACGAGCTGCAGCGGCACCTGCGGACCCACACGGGCGAGAAGCGCTTCG
  CCTGCCCGGTGTGCAACAAACGCTTCATGCGCAGCGACCACCTGAGCAAGCACGTGAAGACACACAGTGG
  TGGCGGCGGCTCGGCGGGCTCCGGCGGCGGCAAGAAAGGCAGCGACACCGACAGCGAGCACAGCGCGGCG
  GGAAGCCCTCCTTGCCACTCCCCTGAGCTGTTGCAGCCTCCAGAGCCGGGCCACCGCAACGGCCTGGAGT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC082582
• Insert Size: 1332 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC082582, AAH82582
• RefSeq Size: 4210 bp
• RefSeq ORF: 1331 bp
• Locus ID: 320145
• Cytogenetics: 12 F2
• Gene Summary: Transcription factor which plays a key role in limb development. Positively regulates FGF8 expression in the apical ectodermal ridge (AER) and contributes to limb outgrowth in embryos.[UniProtKB/Swiss-Prot Function]