D230025D16Rik (BC006874) Mouse Untagged Clone

CAT#: MC218026

D230025D16Rik (untagged) - Mouse RIKEN cDNA D230025D16 gene (cDNA clone MGC:6480 IMAGE:2646515), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: D230025D16Rik
• Synonyms: MGC6480
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC006874
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGACCTGGAGGTGGTGCCTGAACGCTCTCTGGGGAACGAGCAATGGGAATTCACGCTGGGAATGC
  CTCTGGCGCAGGCGGTCGCCATTCTTCAGAAGCACTGTCGCATCATCAGAAACGTCCAGGTTCTCTACAG
  TGAGCAGTCTCCTCTAAGCCATGACCTCATCCTTAACCTGACTCAGGACGGGATCACACTACTGTTTGAT
  GCTTTCAACCAGAGACTCAAGGTGATTGAAGTTTGTGAGTTGACAAAAGTAAAGTTAAAGTATTGTGGAG
  TGCATTTTAACTCTCAGGCCATCGCTCCTACCATTGAGCAAATTGACCAGTCTTTTGGCGCAACCCATCC
  TGGAGTGTACAACTCTACTGAGCAGCTCTTCCACCTCAACTTCCGAGGACTGTCTTTCTCTTTTCAGTTA
  GACTCGTGGACGGAGGCTCCCAAGTATGAGCCCAATTTTGCCCATGGTCTAGCTTCTCTTCAGATACCCC
  ATGGAGCAACTGTAAAACGAATGTACATCTATAGTGGGAACAGCCTCCAGGATACCAAGGCTCCTGTGAT
  GCCCTTGAGCTGTTTCCTGGGGAATGTATATGCTGAAAGTGTAGATGTTCTTCGAGATGGAACTGGACCC
  TCAGGGTTGCGACTTCGCTTGCTCGCTGCAGGTTGTGGACCTGGAGTGTTAGCAGATGCCAAAATGAGAG
  TATTTGAGCGTGCGGTGTATTTTGGTGATTCTTGCCAAGATGTTCTTAGCATGCTTGGCTCTCCACACAA
  AGTCTTCTATAAATCTGAAGACAAGATGAAAATTCACTCTCCTTCCCCTCATAAACAAGTTCCGTCCAAG
  TGCAACGACTACTTTTTTAACTATTTTACTCTCGGAGTGGACATCCTGTTTGATGCAAATACACACAAAG
  TGAAGAAGTTTGTCCTGCATACCAATTACCCTGGGCATTATAATTTTAACATTTACCACCGCTGTGAGTT
  CAAGATCCCACTGGCCATAAAGAAAGAAAATGCAGGTGGTCAGACTGAAATATGCACAACCTACAGCAAG
  TGGGACAGCATCCAGGACCTTCTGGGCCATCCTGTGGAGAAGCCTGTTGTCTTGCACAGGTCCTCATCCC
  CAAATAACACCAATCCGTTTGGCTCCACATTCTGCTTTGGTCTTCAGCGGATGATCTTTGAGGTCATGCA
  GAACAACCACATTGCCTCAGTGACCCTGTATGGCCCCCCCAGGCCTGGTGCCCACCTGAGAACAGCAGAG
  CTGCCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC006874
• Insert Size: 1269 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC006874, AAH06874
• RefSeq Size: 2847 bp
• RefSeq ORF: 1268 bp
• Locus ID: 234678
• Cytogenetics: 8 D3
• Gene Summary: Plays a regulatory role in autophagic activity. In complex with BCAS3, associates with the autophagosome formation site during both non-selective and selective autophagy.[UniProtKB/Swiss-Prot Function]