Hhat (BC008159) Mouse Untagged Clone

CAT#: MC218004

Hhat (untagged) - Mouse hedgehog acyltransferase (cDNA clone MGC:11697 IMAGE:3963716), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hhat
• Synonyms: MGC11697, Skn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC008159
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCGGGTGGGAACTGACTCTCTGCCTGCTTGTTTCCTTGGGTTTCCACTTCCGCTCTTTCTATG
  AAGTTTACAAAGTCTCCAGAGAGCATGAAGAGGAACTGGATCAAGAATTTGAGCTGGAGATGGACACTCT
  ATTTGGAGGACTGAAGAAGGACCCGACTGATTTTGAGTGGAACTTCTGGATGGAATGGGGGAAGCGGAGG
  CTGGTGTGGCTCTTCATTGGCCATATGGCTGTGTCTCAGTTGGCCACGTTACTCACAAAGAAGCACAGAC
  CCTGGATTGTCATGGTCTATGGAATGTGGGCCTGCTGGTGTGTGCTGGGGGCGCCAGGCGTGGTCATGGT
  CCTTCTCCACAGCACCATCGCGTTCTGCGTGGCTCAGTTCCGATCCGTGCTCCTGTCCTGGCTGTGTTCT
  CTCCTTCTGCTCTCCACCCTAAGGCTGCAAAGTGTGGAGGAAGTTAAGAGAAGGTGGTACAAGACAGAGA
  ACGAGTATTACCTGCTCCAGTTTACGCTCACTGTTCGCTGCCTGTACTACACCAGCTTCAGCCTGGAGCT
  GTGCCGGCAGCCTCCGTCTGCACAACCTACTCCATCTGCGCAAGGTGCCTCCCATTCCTACCCGTGGCTG
  CTGACCTACGTCTTCTATTATCCCGTCTTCCACAACGGGCCCATCCTTAACTTCCCGGAGTTCTTCAGAC
  AGATGCAGCAGCCAGAGCTCAACTCTCTGCAGCACAGCCTCTGCATTGTAGCCAAGGGCCTGGGTCGTCT
  GCTCTGCTGGTGGTGGCTGGCTGAACTGATGGTACACCTCATGTACATGCATGCCCTCTACAGCAGCGCC
  CCCCTCCTGGAGAGCGTTTCCTGCTGGACCTTAGGAGGACTGGCGCTGGCCCAAGTGCTCTTCTTCTATG
  TGAAGTACCTGGTGCTCTTTGGTGTCCCGGCTCTGCTCATGCGCCTGGATGGACTCACGCCACCACCTCT
  TCCCCGCTGCGTGAGCACCATGTTCAGTTTTACCGGGATGTGGAGGTATTTTGATGTTGGACTACACAAT
  TTCTTAATCAGGTATGTGTACATCCCACTGGGCGGGTCCCAGCATGGCCTGTTGGGGACACTGCTTTCTA
  CTGCAACAACGTTTGCGTTCGTGAGCTACTGGCATGGTAGCTATGAGGACCTCTGGTGCTGGGCAGCGCT
  CAACTGGCTGGGAGTCACTGTGGAGAGTGGGGTCCGGAGGCTGCTAGAGACACCCTGTGTCCGGGAGACT
  TTGGCCCGGCACCTCTCCCCACAAGCCCACCATCGACTGCACGCTCTGCTTGCCGCCTGCTCTACCTCCA
  TGCTCATCCTCTTCAACCTGGTGTTCCTCGGGGGAATTCAAGTTGGGAAAACCTACTGGAACAGGATCTT
  CCTGCAAGCAGCCTTCAGATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC008159
• Insert Size: 1422 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC008159, AAH08159
• RefSeq Size: 1832 bp
• RefSeq ORF: 1421 bp
• Locus ID: 226861
• Cytogenetics: 1 H6
• Gene Summary: Catalyzes N-terminal palmitoylation of SHH; which is required for SHH signaling during limb development. May bind GTP.[UniProtKB/Swiss-Prot Function]