Dmrt2 (BC027669) Mouse Untagged Clone

CAT#: MC218000

Dmrt2 (untagged) - Mouse doublesex and mab-3 related transcription factor 2 (cDNA clone MGC:41586 IMAGE:1248080), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dmrt2
• Synonyms: Terra
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC027669
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAAGAGAAGAGCCTTTGCTGACAAGGAGTTGGAGAACATTATGCTGGAGAGAGAATATAAAGAGA
  GGGAGATGTTAGAAACATCTCAAGCTGCTGCCTTATTCCTGCCCAACCGTATGGTGCCTGGACCCGAGTA
  CAGTTCCTACAAAGGTACCTACAGCCCCACTGCAGGAGAGCTGCCGAGCAAGGACTTCTGTAACTTTTTA
  CCTACCTGCCTTGATCTCACCATGCAGTATTCAGGGTCTGGGAATATGGAACTTATTTCTTCTAACGTCA
  GTGTGGCCACAACTTACAGACAATATCCCCTGTCCTCACGATTTTTAGTTTGGCCCAAGTGCGGTCCCAT
  TAGTGACACCCTTCTCTACCAGCAATATCTGTTAAATGCTACCACTTCTGTCCAAGCTCTGAAGCCGGGG
  ACCGGCTGGGACTTGAAAGGAACCCGAGTTCAGGATGGGCTTAGCGCAGAGCAAGACATGATGCCACCCA
  AACTGGAAGGCTCTCTGGTGCTGCCCCATCTGCCAGAGGTCCCAGCCTCGAGAACAGACCTTCAGGTTCA
  CCAGGTGGTTCCAGAGAGGTCTGCCTTCTCCCCACCCGGTCGGAATTTCTCTCCCATTGTTGATATGGAC
  TGCCTGGCAGCTCAAGGGCACGTCTTAACCAAGCTCAGCAAAGAAAACACTAGACCTTCTCTGCCACTTA
  AAACTAATCCATTCCACTCAGTATTCCAGCAGACGCTCAGCGACAAATCAGGCCCTGAGTTGAACGCACC
  ATTTGTCAAAGAAGCCTTTGAAGAGACCCCAAAGAAACACAGAGAGTGTTTGGTCAAGGAGAGCCAGAAG
  TACACATTTACAATAGACAGATGCGCAAAAGACCTCTTTGTAGCCAAACAAGTTGGAACGAAACTTTCGG
  CGAATGAGCCACTGTCGTTCTCTGTCGAATCTATTCTTAAGAGGCCTTCATCTGCCGTCACTCACGTCTC
  TCAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC027669
• Insert Size: 987 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027669, AAH27669
• RefSeq Size: 1304 bp
• RefSeq ORF: 986 bp
• Locus ID: 226049
• Cytogenetics: 19 C1
• Gene Summary: Transcriptional activator that directly regulates early activation of the myogenic determination gene MYF5 by binding in a sequence-specific manner to the early epaxial enhancer element of it. Involved in somitogenesis during embryogenesis and somite development and differentiation into sclerotome and dermomyotome. Required for the initiation and/or maintenance of proper organization of the sclerotome, dermomyotome and myotome. Is not required for sex determination and/or differentiation in embryonic development. Also not involved in symmetric somite formation and hence does not regulate the laterality pathway that controls left-right asymmetric organ positioning.[UniProtKB/Swiss-Prot Function]