Unc84b (BC098208) Mouse Untagged Clone

CAT#: MC217993

Unc84b (untagged) - Mouse unc-84 homolog B (C. elegans) (cDNA clone MGC:106463 IMAGE:6827666), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Unc84b
• Synonyms: SUN2, C030011B15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC098208
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAGACGAAGCCAGCGCCTCACTCGCTACTCTCAGGATGATAACGATGGCGGCAGCAGCAGCAGTG
  GTGCGAGCTCCGTGGCAGGAAGCCAGGGCACCGTGTTTAAAGACAGTCCTCTCAGGACTTTGAAGAGGAA
  ATCCAGCAACATGAAGCACCTGTCCCCAGCTCCACAGCTGGGCCCCTCCTCTGACTCCCACACCTCCTAC
  TACAGCGAGTCTGTGGTTCGAGAGTCCTACATCGGCAGCCCCCGGGCTGTGTCCCTCGCCAGGAGTGCCC
  TCCTGGATGACCACCTACACAGTGAGCCCTACTGGAGCGGGGACCTTCGGGGGAGGAGGAGGAGAGGAAC
  AGGTGGTTCTGAGAGCAGCAAGGCCAATGGGCTCACCGCGGAGAGCAAGGCCTCAGAAGACTTTTTCGGA
  TCTTCCTCAGGCTATTCTTCAGAGGATGACCTTGCAGGCTACACGGACTCAGACCAGCACAGCTCGGGGT
  CCAGGTTAAGGAGTGCAGCATCTCGGGCCGGCTCCTTTGTCTGGACTCTGGTCACTTTTCCAGGCCGCCT
  CTTTGGTCTTCTCTACTGGTGGATTGGCACCACCTGGTACCGCCTGACAACTGCTGCCTCCCTCCTGGAT
  GTCTTCGTCCTAACCAGGCACTTCTCGCTGAACCTGAAGAGTTTTCTGTGGTTCCTTCTGCTCTTGCTAC
  TCCTGACTGGTCTGACCTACGGTGCTTGGCATTTTTACCCCTTAGGGCTGCAGACATTGCAACCCGCTGT
  GGTCTCCTGGTGGGCAGCAAAAGAGAGCAGGAAGCAGCCAGAGGTGTGGGAATCCAGAGACGCCTCCCAG
  CACTTCCAGGCTGAGCAGCGCGTTCTCTCCCGGGTTCACTCTCTGGAGCGGCGTCTGGAAGCCCTTGCTG
  CAGACTTTTCCTCCAACTGGCAGAAGGAGGCCATACGGCTGGAACGCCTGGAGCTGCGGCAGGGGGCTGC
  TGGCCATGGAGGAGGCAGTAGCCTGAGCCATGAAGATGCCCTGTCTCTCCTAGAAGGGTTGGTGAGCCGC
  CGCGAGGCTACCCTGAAGGAGGACTTGCGCAGGGACACAGTGGCTCATATCCAGGAAGAATTGGCTACCC
  TGAGGGCAGAGCATCACCAAGACTCGGAAGATCTCTTCAAGAAGATCGTCCAGGCCTCTCAGGAGTCCGA
  AGCCCGAGTCCAGCAGCTGAAGACAGAATGGAAAAGCATGACCCAGGAGGCCTTCCAGGAGAGCTCTGTG
  AAGGAGCTGGGACGGCTGGAAGCCCAGCTGGCCAGCCTGCGGCAGGAGCTGGCTGCCCTGACTCTGAAGC
  AGAACTCGGTGGCAGATGAAGTGGGCCTGCTGCCACAGAAGATCCAGGCTGCCAGGGCTGATGTGAGCGG
  GAAGTACCCAGAGCCCTACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC098208
• Insert Size: 1422 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC098208, AAH98208
• RefSeq Size: 4142 bp
• RefSeq ORF: 1421 bp
• Locus ID: 223697
• Cytogenetics: 15 E1
• Gene Summary: As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Required for nuclear migration in retinal photoreceptor progenitors implicating association with cytoplasmic dynein-dynactin and kinesin motor complexes, and probably B-type lamins; SUN1 and SUN2 seem to act redundantly. The SUN1/2:KASH5 LINC complex couples telomeres to microtubules during meiosis; SUN1 and SUN2 seem to act at least partial redundantly. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. May also function on endocytic vesicles as a receptor for Rab5-GDP and participate in the activation of Rab5.[UniProtKB/Swiss-Prot Function]