Pomt2 (BC052045) Mouse Untagged Clone

CAT#: MC217986

Pomt2 (untagged) - Mouse protein-O-mannosyltransferase 2 (cDNA clone MGC:62419 IMAGE:6402328), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pomt2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052045
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCCGGCCATAGGCGGTGGCCTAGCGGGCTCCGAGCTGCGTCCCCGTAGGGGCCGCTGTGTGCCGC
  AGGCTGCCAGAGCCGTGAGCCGAGACGTGGTCCCCCAGGCTGCAGCACGGAAACTTAAACGGCCTGCTTG
  GAGCTCGCGGCGCTTCCAGGCGGCAGGGTGGTGGGCCACGTTGGCTGTGGTGACGCTGCTGTCCTTTGCC
  ACCCGCTTTCACCGGCTGGACCAGCCTGCTCACATCTGTTGGGATGAGACTCACTTTGGAAAAATGGGAA
  GTTACTATATTAACCGCACCTTTTTCTTTGATGTGCATCCACCACTGGGAAAGATGCTGATCGGTCTTGC
  TGGCTACCTGAGCGGGTATGATGGTACCTTTCTGTTCCAGAAGCCTGGGGACAGATACGAGCACCACAGC
  TACATGGGAATGAGAGGATTCTGTGCTTTCCTGGGTTCCTGGCTGATCCCCTTTGCCTACCTCACCGTAC
  TGGATCTGTCCAAGTCCTTCCCAGCAGCACTGCTCACCGCTGCCCTTCTCACCTTTGACACAGGATGCCT
  CACTCTATCCCAGTACATCCTCCTTGACCCCATCCTGATGTTCTTCATCATGGCGGCCATGCTGAGCATG
  GTCAAGTACAACTCCTGTGCTAACAGGCCTTTCTCTGCCCCCTGGTGGTTCTGGCTCAGCCTGACTGGCA
  TTAGTCTCGCTGGTGCTTTAGGGGTCAAATTTGTTGGCCTCTTTATCATCGTGCAAGTGGGGTTGAACAC
  TATTTCAGACCTCTGGCATCTGTTTGGAGACCTCAGCCTTTCACTGGTGACTGTGGGGAAGCACCTAACT
  GCTCGCATCCTGTGCCTCATAGTGCTGCCTTTGGTGCTCTATGTGACCATTTTTGCTGTTCATGTCATGG
  TGCTGAATAAAAGTGGTCCTGGTGATGGCTTCTTCAGTTCTGCCTTTCAAGCCCGGCTTTCAGGGAACAG
  CCTTCACAATGCTTCTATCCCTGAACACCTGGCGTATGGCTCTGTAATCACTGTGAAGAATCTCCGGATG
  GCCATTGGCTACCTGCACTCTCATAGGCACCTCTACCCTGAGGGCATTGGTGCTCGCCAGCAGCAGGTTA
  CCACCTATTTGCATAAGGACTACAACAACTTGTGGATTATCAAGAAGTATAATGCCAACACAGATCCCCT
  AGACCCTTCATTCCCAGTGGAGTTTGTCAGACATGGAGACATCATACGACTAGAACACAAAGAAACTACT
  CGGAACCTGCACAGTCACTATCATGAGGCTCCCCTGACCCGGAAGCACTATCAGGTCACTGGCTATGGCA
  TAAATGGGACAGGGGACTCAAATGACTTCTGGCGGATTGAAGTTGTAAATAGAAAATTTGGGAACCGGAT
  CAAGGTACTGAGAAGTCGAATTCGCCTCATCCATTTGGTCACAGGTTGTGTCCTGGGATCGTCAGGAAAG
  ATTCTGCCTAAGTGGGGCTGGGAGCAGTTGGAAGTTACCTGTACTCCGTACCTAAAAGAAACCACTAACT
  CCATTTGGAACATAGAAGAGCATATCAATCCCAAATTGCCAAACATCAGCCTGGATGTGCTGCAGCCCAG
  TTTTCCTGAGATCTTGCTGGAGTCTCACATGGTCATGATCCGGGGAAATAATGGCCTCAAACCCAAGGAC
  AATGAGTTCACATCCAAGCCCTGGCACTGGCCTATCAATTATCAGGGCCTGCGCTTCTCAGGGGCCAATG
  ACACGGACTTCCGAGTCTATCTGCTCGGCAACCCTGTGAGTATCCCAGCTCTGAGTGCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC052045
• Insert Size: 1812 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052045, AAH52045
• RefSeq Size: 2800 bp
• RefSeq ORF: 1811 bp
• Locus ID: 217734
• Cytogenetics: 12 D2
• Gene Summary: This gene encodes an integral membrane protein that belongs to the dolichyl-phosphate-mannose-protein mannosyltransferase family. The encoded enzyme is found in the membrane of the endoplasmic reticulum. This protein is a component of the protein O-mannosyltransferase enzyme complex which is involved in modification of the protein alpha-dystroglycan. Mutations in the human gene are a cause of different forms of muscular dystrophy-dystroglycanopathy (MDDG), type A2 (also known as Walker-Warburg syndrome), type B2 and type C2 (also known as limb-girdle muscular dystrophy). [provided by RefSeq, Sep 2015]