Tle6 (BC048832) Mouse Untagged Clone

CAT#: MC217953

Tle6 (untagged) - Mouse transducin-like enhancer of split 6, homolog of Drosophila E(spl) (cDNA clone MGC:55024 IMAGE:4506029),, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tle6
• Synonyms: Grg6, 1810057E06Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC048832
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTATTGGTGTTGGGGCTGAAGGGGACAGTTGGCCATGTCATGTGTCTCATGAGGCCCCAATGGGCT
  CTGCCCAGACCACAGAGAACTCAGCTAAGGAAGAAGACAAACAGGTCCCAGAGTCTGCAGCCCTCCAGCA
  CCCCAAGTTCAAAAGCACCCCGGGGCCTCAGCTGCCCACCAGACGTCGGTTCCTCTCAGAGAGTGATGAG
  CTCCAGGACCCACAGCCGGTCTGGGACGCGGAGCCCCAGTTCTGTCAAGGCTTCCTGATCCAGGGACTCT
  GGGAACTTTTCATGGACAGCCGCCAAAAGAACCAACAGGAACACGGGGGTGAGGATTCCTCACAGGAAAG
  CAAAGATTCAGGCCTGTGTGACTTTAAACCAGAACCTCAGCCCAGGCACAGAAACAGCCTGAGTGACTCT
  GCCGACCCCTTCCTGATCAAGTCTCCCTCAGCTCTTCTAGATTATTACCAAGAAGACGTCAGTCGGCCCC
  AACCTGAGACGCAGGAATCTTCTGGAAGAGCTGATAAATTTCTGAAGCCCTTGTCCTGGGGCTCTGAAGT
  CTTAGAGAGCAGCTGTAATCAGCCTAGCACAGCACTCTGGCAATTAGAGAGGTTCACTGTCCCCCAAGCA
  CTGCAGAAGGTGCGTGTGCTGAAGCACCAGGAACTGCTATTGGTGGTAGCTGTGAGCTCCTTCACGCGGC
  ACGTCTTCACCTGCAGCCAGAGCGGCATCAAGGTGTGGAACCTCGTGAACCAGGTGGCCGAGGACAGAGA
  CCCAGAAAGCCACTTGAAATGCAGCGTCCAGGACAACAAGGTGTACCTGCGCACCTGCCTGCTGTCCTCC
  AACAGCAGGACCCTGTTCGCGGGTGGATACAACTTACCTGGCGTGATCGTGTGGGACCTGGCAGCCCCAT
  CCCTGTATGAGAAGTGCCAGCTGCCCTGCGAGGGCCTGTCCTGCCAGGCACTGGCCAACACAAAGGAAAA
  CATGGCCCTTGCCGGCTTCACAGATGGCACGGTCAGGATATGGGACCTACGGACTCAGGAAATAGTCAGG
  AACCTCAAAGGCCCTACCAATTCGGCCAGGAACCTTGTGGTCAAAGATGACAACATCTGGACAGGGGGTC
  TGGACGCCTGCCTGCGATGCTGGGACCTGCGGATGGCCAAGGTGTCACTGGAGCATCTGTTCCAGTCCCA
  GATCATGAGCCTGGCTCACAGTCCGACCGAAGACTGGCTGCTGCTGGGCCTGGCCAATGGCCAGCACTGC
  CTGTTCAACAGCAGGAAGAGGGACCAGGTGCTCACTGTGGACACCAAGGACAACACCATCTTAGGCCTCA
  AGTTCTCCCCAAATGGCAAGTGGTGGGCGAGCGTCGGTATGGGCAACTTCATCACGGTCCACAGCATGCC
  CACCGGAGCAAAACTGTTCCAGGTCCCCGAGGTTGGCCCTGTCAGATGCTTCGACATGACTGAGAACGGC
  AGGCTCATCATCACAGGATCCAGGGACTGTGCCTCGGTGTACCACATCAAGTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC048832
• Insert Size: 1527 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC048832, AAH48832
• RefSeq Size: 1897 bp
• RefSeq ORF: 1526 bp
• Locus ID: 114606
• Cytogenetics: 10 C1
• Gene Summary: As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions.[UniProtKB/Swiss-Prot Function]