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B3gnt5 (NM_001159408) Mouse Untagged Clone

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SKU
MC217949
B3gnt5 (untagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant 3, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free DDK
AAV Particle:
DDK
  $503.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol B3gnt5
Synonyms beta3GnT5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC217949 representing NM_001159408
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGACTGTTTGTTAGCAGAAGAGTCAAAAGATGGAAAATTTTTCACTTTTTTGTCACTTGTTTTATAT
TAAGCTTCATGGTTTTTTGGAGCCCAATCAATAATTACATCATGAGCCATATGAAGTCCTACTCCTACAG
ATACCTCGTAAATAGCTATGGCTTTGTAAACAATTCCCTGTCTCTCAAGCACAGCTCTGTGCAGCCTCAC
TACCCATACTTGATCAACCACAGAGAGAAGTGTCAGGCTCAAGATGTCCTCCTCTTACTGTTTATAAAGA
CTGCCCCTGAAAACTATGGCCGACGTTCTGCAATCAGAAAAACGTGGGGCAATGAGAACTATGTTCAGTC
TCAACTCAATGCCAACATCAAAATTCTGTTTGCATTAGGAACTCCTGGTCCACTGAAGGGAAAAGAACTG
CAAAAAAGACTAATCGGGGAAGATCAAGTGTACAAGGATATAATTCAGCAAGATTTCATTGATTCTTTCC
ACAATCTTACTTCTAAATTCCTTCTTCAGTTCAGCTGGGCAAATACCTTTTGTCCACATGCCAAATTCCT
GATGACTGCTGATGATGATATATTTATCCACATGCCAAATCTCATTGAATATCTTCAAGGGCTAGAGCAG
ATTGGAGTTCGAGACTTTTGGATTGGTCACGTTCATCGAGGTGGCCCTCCTGTTAGGGATAAAAGCAGCA
AATACTATGTTCCCTATGAAATGTACAAGTGGCCAGCCTACCCTGACTATACAGCTGGTGCTGCCTATGT
TGTCTCCAGAGATGTAGCTGCCAAAATCTATGAGGCATCGCAGACACTGAATTCCAGTATGTACATAGAT
GATGTATTCATGGGCCTCTGTGCCAATAAAGTGGGAATCTTGCCACAGGACCATGTATTTTTCTCTGGGG
AAGGGAAAATTCCTTATCACCCCTGCATCTATGAAAAGATGATGACATCTCACGGACACTTACAAGATCT
GCAGGACCTCTGGATAGAGGCTACACATCCTAAAGTAAAGAACATTTCAAAAGGGTTTTTTGGTCAAATA
TACTGCAGGTTAATTAAGATAGTTCTTCTCTGCAGACTGACTTACAGGAATTCATACCCTTGTTGGGCTG
CATTTGCTTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001159408
Insert Size 1131 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001159408.1, NP_001152880.1
RefSeq Size 4669 bp
RefSeq ORF 1131 bp
Locus ID 108105
UniProt ID Q8BGY6
Cytogenetics 16 A3
Summary Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc(4)Cer; paragloboside), resulting in the synthesis of Lc(3)Cer and neolactopentaosylceramide (nLc(5)Cer), respectively. Plays a central role in regulating neolacto-series glycolipid synthesis during embryonic development.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All three variants encode the same protein.
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Documents
Resources
"NM_001159408" in other vectors (3)
SKU Description Size Price
MR205836 B3gnt5 (Myc-DDK-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant 3 10 ug
$289.00 $457.00
MR205836L3 Lenti ORF clone of B3gnt5 (Myc-DDK-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant 3 10 ug
$757.00
MR205836L4 Lenti ORF clone of B3gnt5 (mGFP-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant 3 10 ug
$757.00

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