Trim23 (BC059017) Mouse Untagged Clone

CAT#: MC217929

Trim23 (untagged) - Mouse tripartite motif protein 23 (cDNA clone MGC:69677 IMAGE:6848690), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim23
• Synonyms: 6330516O20Rik; AI450195; Arfd1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC059017
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCCTCGCTGTAAACAAGCCCGGAGCCGGAGTAGACAGTGGGCGGCAGGGCAGCCGGGGGACGG
  CTGTGGTGAAGGTTCTGGAGTGTGGGGTTTGTGAAGATGTCTTCTCTTTGCAAGGAGATAAAGTTCCTCG
  CCTCCTCCTCTGTGGCCACACAGTCTGTCATGACTGTCTTACTCGCTTGCCTCTGCATGGAAGAGCAATA
  CGTTGCCCATTTGATCGACAAGTAACAGACCTAGGAGACTCAGGTGTATGGGGTTTGAAAAAGAACTTTG
  CTTTACTGGAGCTTTTAGAACGTTTGCAAAATGGGCACATTGGTCAGTATGGAGCTGCAGAAGAGGCCAT
  TGGGACATCTGGAGAGAGCATCATTCGCTGTGATGAAGATGAAGCTCACGTTGCATCTGTATATTGCACT
  GTGTGTGCAACTCACTTGTGCTCAGACTGTTCTCAAGTTACTCATTCTACAAAGACATTAGCAAAGCATA
  GGCGTGTGCCTCTAGCTGATAAACCTCATGAGAAAACCATGTGCTGTCAGCACCAGGTGCATGCCATTGA
  GTTTGTTTGCTTGGAAGAAGGTTGTCAAACTAGTCCACTCATGTGCTGTGTCTGCAAAGAATATGGAAAG
  CACCAAGGTCACAAGCATTCAGTACTGGAACCAGAAGCTAATCAGATCCGAGCATCAATTTTAGACATGG
  CTCACTGCATACGGACCTTCACTGAGGAAATCTCAGATTATTCCAGAAAATTAGTTGGGATTGTTCAGCA
  CATTGAAGGAGGAGAACAAATAGTAGAAGATGGAATTGGAATGGCTCACACAGAACATGTCCCAGGTACT
  GCAGAGAATGCCCGATCATGTGTCAGAGCTTATTTTTCTGATTTACATGAAACTCTTTGTCGTCAAGAAG
  AAATGGCTCTAAGTGTTGTTGATGCCCATGTTCGAGAAAAACTGATTTGGCTTAGGCAGCAACAAGAGGA
  TATGACTATTCTCTTGTCCCAGGTCTCAACAGCCTGTCTCCACTGTGAAAAAACTCTGCAGCAGGATGAT
  TGCAGAGTTGTCTTGGCAAAACAAGAAATCACAAGATTACTAGAAACACTGCAGAAACAGCAGCAGCAGT
  TTACAGAGGTTGCAGATCATATTCAGTTGGATGCCAGTATTCCAGTCACTTTTACAAAGGACAATAGAGT
  TCATATTGGACCCAAAATGGAAATCCGAGTAGTCACATTAGGATTAGATGGTGCTGGAAAAACTACCATT
  TTGTTCAAGTTAAAACAAGATGAATTTATGCAACCTATTCCAACAATTGGTTTTAATGTGGAAACTGTGG
  AATACAAAAATCTAAAATTCACCATTTGGGATGTGGGTGGAAAACACAAATTAAGACCATTGTGGAAACA
  TTATTACCTCAATACACAAGCTGTTGTATTTGTTGTTGATAGTAGTCATAGAGACAGAATTAGTGAAGCA
  CACAGTGAACTTGCAAAGTTGTTAACGGAAAAAGAACTCCGAGATGCCTTACTCTTGATTTTTGCTAACA
  AACAGGATGTAGCTGGAGCACTTTCAGTAGAAGAAATCACTGAACTTCTCAGTCTCCATAAACTATGCTG
  TGGAAGGAGTTGGTATATTCAGGGCTGTGATGCTCGAAGTGGAATGGGGCTCTACGAAGGGTTGGACTGG
  CTGTCCCGGCAACTTGTGGCTGCTGGAGTGTTGGATGTTGCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC059017
• Insert Size: 1725 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC059017, AAH59017
• RefSeq Size: 3385 bp
• RefSeq ORF: 1724 bp
• Locus ID: 81003
• Cytogenetics: 13 D1
• Gene Summary: Acts as an E3 ubiquitin-protein ligase. Plays an essential role in autophagy activation during viral infection. Mechanistically, activates TANK-binding kinase 1/TBK1 by facilitating its dimerization and ability to phosphorylate the selective autophagy receptor SQSTM1. In order to achieve this function, TRIM23 mediates 'Lys-27'-linked auto-ubiquitination of its ADP-ribosylation factor (ARF) domain to induce its GTPase activity and its recruitment to autophagosomes.[UniProtKB/Swiss-Prot Function]