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Tmem175 (NM_001163532) Mouse Untagged Clone

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SKU
MC217887
Tmem175 (untagged) - Mouse transmembrane protein 175 (Tmem175), transcript variant 3, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free DDK
AAV Particle:
DDK
  $503.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Tmem175
Synonyms 3010001K23Rik; AI504381; mTMEM175
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC217887 representing NM_001163532
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCAGGCTCCAGACTGAGGAGCAGGCAGTGGATTCCGAGGGAGACTCATCCCTACACAGGAGAAATG
AGGAGGGGACACAGAGCTCCCACCGCATGCTGGGCTTCAGTGATGCACTGCTGTCCATCATCGCCACTGT
CATGATCCTGCCTGTGACCCACACAGAGATCTCACCAGAACAGCAGTTTGACAAAAGTATACAGAAACTC
CTAGCAACTAGGATCGCTGTCTACCTCATGACATTTCTAATCGTAACTGTGGCCTGGACAGCACATACCA
GATTGTTCCAGGTTGTTGGAAAAATAGATGATACACTTGCCTTGCTCAACCTGGCCTGTATGATGACTAT
CACCCTCCTGCCCTACACATTTTCACTAATGGTAACATTCCCTGATGTGCCCCTGGGCATCTTCCTGTTC
TGCGTGTGTGTGATCGCCATTGGATCTGTGCAGGCAATGATTGTGGGCTATGCCTTCCACTTCCCACATC
TGCTGAATCCACAAATCCAGTGCTCTACACATAGGGATCTATCCCGGCGGCATATCCTGCACCTGGTCCT
CCGTGGCCCAGCACTGTGTTTTGTTGCGGCTGTCTTTTCCCTCTTCTTCTTCCCCTTGTCATACCTGCTG
ATGGTGACTGTCATCTTCCTCCCTCACATCAGCAAGGCTACCACCTGGTGCAAAGACAAGTTGATGGGCC
AAAGGGAGTCTCCAGCTCACGACATGGAGCCCTTCAGTATTGACCTGCATGCGCCCCTCAGCAAAGAGCG
GGTGGAAGCTTTCAGCGACGGTGTCTATGCCATCGTGGCCACACTCCTCATCCTGGACATCTGTGAGGAC
AATGTTCCGGATCCCAAGGATGTGCAAGAGAAATTCAGTGGCAGCCTTGTGGCTGCCCTGGGTGCATATG
GGCCACAATTCTTGGCATACTTCGGCTCCTTCGCCACAGTGGGTCTCCTCTGGTTTGCTCACCATTCACT
CTTCCTGCATGTCCGGAAGGCTACACAGACCATGGGGTTACTCAACATACTATCGCTGGCCTTTGTGGGT
GGCCTTCCTCTGGCCTACCAGCAGACCTCAGCTTTTGCCCGGCAACCCCATGATGAACTTGAGCGTGTGC
GTGTCAGCTGTGCCATCATCTTCTTTGCCAGCATCTTCCAGTTTGCCATCTGGACTACAGCCCTGCTGCA
TCAGACAGAAACACTGCAGCCAGCTGTGCAATTTGGTGGGCAGGAGCATGCTTTCATGTTTGCCAAGCTT
GCACTGTATCCCTGTGCCAGCCTGCTGGCCTTTGCTGCCACCTGCCTACTGAGTCGTTTCAGCACAGCCA
TCTTCCACCTCATGCAGATTTCAGTGCCCTTTGCATTCCTGTTGCTCCGTCTCCTTGTACGCCTTGCCCT
GGCAGGCCTTCAAGTCCTACGGGGCCTCTGGCCACACCATCCCCAACAAGACCAGAGTGAACCTGAGGCA
CAGTCCCAGCTCTTGCCTGACCCCTGCTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001163532
Insert Size 1500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001163532.1, NP_001157004.1
RefSeq Size 2966 bp
RefSeq ORF 1500 bp
Locus ID 72392
UniProt ID Q9CXY1
Cytogenetics 5 F
Summary Organelle-specific potassium channel specifically responsible for potassium conductance in endosomes and lysosomes. Forms a potassium-permeable leak-like channel, which regulates lumenal pH stability and is required for autophagosome-lysosome fusion. Constitutes the major lysosomal potassium channel.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 4. Variants 1, 3, and 4 all encode the same isoform (1).
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Documents
Resources
"NM_001163532" in other vectors (3)
SKU Description Size Price
MR208006 Tmem175 (Myc-DDK-tagged) - Mouse transmembrane protein 175 (Tmem175), transcript variant 3 10 ug
$289.00 $457.00
MR208006L3 Lenti ORF clone of Tmem175 (Myc-DDK-tagged) - Mouse transmembrane protein 175 (Tmem175), transcript variant 3 10 ug
$757.00
MR208006L4 Lenti ORF clone of Tmem175 (mGFP-tagged) - Mouse transmembrane protein 175 (Tmem175), transcript variant 3 10 ug
$757.00

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