Qpct (BC020023) Mouse Untagged Clone

CAT#: MC217871

Qpct (untagged) - Mouse glutaminyl-peptide cyclotransferase (glutaminyl cyclase) (cDNA clone MGC:27858 IMAGE:3491756), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Qpct
• Synonyms: 5730422A13Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC020023
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGCAGCGAAGACAAACGCGTCGTGGGCACTCTCCACCTGCTGCTACTGCAGGCGACCGTCCTGT
  CTCTGACAGCTGGGAATCTGAATCTGGTCTCCGGTGCCTGGACGCAGGAGAAGCACATCATGCAACGAAT
  TCAGAGACTTCAGGCTGAGTGGGTCGTGGAAGTTGACACCTTCCTGAGTAGGACTCCCTATGGCTATCGG
  TCCTTCTCAAATATCATCAGCACTCTTAACCCGGAAGCGAAACGACACCTGGTCCTCGCCTGCCACTACG
  ACTCCAAATATTTTCCTCGATGGGACAGCAGAGTGTTTGTGGGAGCCACGGATTCAGCTGTGCCATGTGC
  AATGATGTTGGAACTTGCCCGTGCCTTAGACAAGAAACTCCATTCCTTGAAGGATGTCTCTGGTTCCAAG
  CCAGATCTCTCACTCCGGCTAATTTTCTTTGATGGTGAAGAGGCTTTTCATCACTGGTCCCCTCAAGATT
  CTCTGTATGGGTCTCGGCACTTAGCTCAGAAGATGGCATCAAGCCCTCACCCTCCTGGATCAAGAGGCAC
  CAACCAACTGGATGGCATGGATCTGTTGGTCTTACTAGATTTAATTGGAGCAGCAAATCCAACATTCCCT
  AATTTTTTCCCCAAGACTACCAGATGGTTTAATAGACTTCAAGCAATTGAAAAGGAACTCTATGAATTGG
  GATTACTCAAGGATCATTCTTTGGAGAGGAAGTATTTTCAGAATTTTGGCTATGGAAATATTATCCAGGA
  TGACCACATTCCATTTTTAAGAAAAGGTGTCCCAGTTCTTCACCTGATAGCTTCTCCTTTCCCTGAAGTC
  TGGCACACCATGGATGACAATGAAGAAAATCTACATGCGTCAACCATTGACAATCTCAACAAAATCATTC
  AAGTCTTTGTGTTGGAATATCTTCACTTGTAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATA
  TCCTGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: BC020023
• Insert Size: 942 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC020023, AAH20023
• RefSeq Size: 1486 bp
• RefSeq ORF: 941 bp
• Locus ID: 70536
• Cytogenetics: 17 E3
• Gene Summary: Responsible for the biosynthesis of pyroglutamyl peptides. Has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue (By similarity).[UniProtKB/Swiss-Prot Function]