Wdfy1 (BC025226) Mouse Untagged Clone

CAT#: MC217861

Wdfy1 (untagged) - Mouse WD repeat and FYVE domain containing 1 (cDNA clone MGC:32262 IMAGE:5011404), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wdfy1
• Synonyms: 1700120F24Rik, WDF1, FENS-1, Jr1, ZFYVE17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC025226
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTTTATCAAGACCTATCCAGCCCACCAGAACCGCGTGTCTGCTATCATCTTCAGCCTGTCTGCCG
  AGTGGGTGATCAGCACTGGCCACGACAAGTGTGTGAGCTGGATGTGCACGCGCAGCGGGAATATGCTTGG
  CCGCCACTTCTTCTCGTCGTGGGCTTCCTGTTTGCAGTATGATCTGGACACTCAGCACGCCTTTGTTGGC
  GATTACTCGGGACAGATCACCCTGCTGAAGCTGGAGCAGAACACTTGTTCAGTTATCACGACCCTCAAGG
  GACATGAAGGTAGCATCGCCTGCCTTTGGTGGGACCCTATCCAACGGTTACTCTTCTCGGGAGCATCCGA
  CAACAGCGTCATCATGTGGGACATCGGGGGACGGAAAGGCCGGACACTCCTCCTTCAAGGCCACCATGAC
  AGAGTGCAGTCACTGTGCTACCTGCAGCTCACGAGGCAGCTCGTCTCCTGCTCGGCAGATGGTGGCATTG
  CAGTGTGGAACATGGATGTTAGCAGAGAAGAGGCTCCTCAGTGGTTAGAAAGCGACTCCTGTCAGAAGTG
  TGAGCAGCCCTTCTTCTGGAATATAAAACAGATGTGGGACACAAAGACCCTGGGCTTGAGGCAGCATCAC
  TGCAGGAAGTGTGGGCAGGCCGTCTGTGGGAAGTGCAGCAGCAAGCGGTCCAGTTACCCCGTCATGGGTT
  TTGAGTTCCAGGTTCGAGTTTGTGATTCCTGTTACGACTCCATCAAAGATGAGGACCGGACTTCTCTAGC
  AACATTTCATGAAGGAAAACATAACATTTCCCATATGTCCATGGACATTGCAAGGGGACTGATGGTGACC
  TGTGGGACAGATCGTGTTGTGAAGATATGGGATATGACTCCTGTGGTGGGCTGCAGCCTGGCAACTGGAT
  TTTCTCCACACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC025226
• Insert Size: 924 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC025226, AAH25226
• RefSeq Size: 1363 bp
• RefSeq ORF: 923 bp
• Locus ID: 69368
• Cytogenetics: 1 C4
• Gene Summary: Positively regulates TLR3- and TLR4-mediated signaling pathways by bridging the interaction between TLR3 or TLR4 and TICAM1. Promotes TLR3/4 ligand-induced activation of transcription factors IRF3 and NF-kappa-B, as well as the production of IFN-beta and inflammatory cytokines (PubMed:25736436).[UniProtKB/Swiss-Prot Function]