4432405B04Rik (BC058375) Mouse Untagged Clone

CAT#: MC217847

4432405B04Rik (untagged) - Mouse RIKEN cDNA 4432405B04 gene (cDNA clone MGC:66869 IMAGE:6828178), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 4432405B04Rik
• Synonyms: 4432405B04Rik; Tect2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC058375
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTAGTTTCAGCCTCCCACAATGCATCCTGCTTAGCACATCTGCTCATCCAGGTGGAGATTTATC
  CCAACACATCTGTAACCCACAACGCATCAGAGAACATGACTGTCATTCCTAACCAAGTCTATCAGCCCCT
  GGGTCCCTGCCCGTGTGACTTAACAGCCAAGGCCTGTGACATTCAATGCTGCTGTGACCAGGACTGTCAG
  CCAGAAGTAAGAGAGCTGTTTGCACAGTCCTGCTTCTCCGGCGTGTTTGGAGGGCACGTCAGCCCTCCGT
  CCCATCATCACTGCGCTGTCAGCACTACTCACCAGACTCCTGACTGGTTTCCCCTCCTGTGTGTGCAGTC
  GCCACCCTCTACCTCGCCGTTCCTTGGTCACTTCTACCACGGTGCCACTTCCCCCAGACATTCCCCCGGC
  TTTGAAGCCCATTTGCACTTCGACCTAAGAGACTTCGCGGATGCCAGTTACAAGCAAGGAGATCCCATTA
  TGACCACGGAGGGGTATTTTACCATCCCCCAGGTGTCTCTAGCTGGGCAGTGTCTACAGGATGCTCCTGT
  GGCTTTCCTGAGGAATTTTGACAGTGTATGTACCATGGACCTGGAAGTGCACCAAGGACGAGATGAAATT
  GTCCTCGAGAACATGAAAATCCGCACAACAGGAGGTCCGGTCACACCAACAGTAACCTATGAGGAAGCCA
  TTGACCTGGACAAGTTCATCACCAGCCCGGACACAGTTTTAAGTGTCGGATCGGCCCCCAGAAACGTGAA
  TGTGGAAGAACATTATGTCTTCAGATGGCAAAATAATTCCATCAGCGGCCTAGACATCACAGTCATCAGG
  GCAGAAATCAGTGCCCAGCAGAGAGGAATGATGACCCAGAGATTCACAGTGAAGTTTTTAAGCCATCACA
  GTGGTGGTGAGAAGGAATTTTCTGGAAATCCAGGTTACCAGCTTGGCAAGCCGGTCCGAGCCCTGCATAC
  CGCTGGGATGAACGTCAGCACACTGCACCTCTGGCAGCCAGCTGGAAGGGGCCTGTGCACAGCAGCCGCT
  CTCAGACCCATTTTGTTTGGAGAAAATGCCTTCTCTGGCTGCCTGCTGGAAGTTGGGATTAAGGAGAACT
  GTACCCAGCTCAGAGAGAATGTCCTTCAGAGACTGGATTTGCTGACACAAGCAACGCACGTGGCGAGGAG
  AGGCAACTCGGACTACAGTGATCTGAGTGACGGCTGGCTGGAGATCATACGTGCCGAAGCCCCTGATACA
  GGTGCCGACCTGCCCTTGAGCAGCGTGAATGGCGTGTGCCCAGAAGTTCCAGCCCGGCTCTCCATCCGCA
  TTCTCACTGCTGAAGCTGGCTCTGTGGAAGGGGTGGCTCAGCGGGAGATCCTGGCTGTGGAGACAAGGTT
  TTCCACAGTGACCTGGCAGTACCAGTGTGGGCTCACTTGTGAGGACAAGGCCGACCTTCTCCCTCTCAGT
  GCCTCTGTTGAGTTCATTAATGTCCCTGCGCAGATGCCCCACCCCCCTACGAGATTCCAGATCAACTTCA
  CAGAGTACGACTGTACCCGGAATGAGCTGTGTTGGCCCCAACTTCTGTACCCTCTGACCCAGTATTATCA
  AGGGGAGCCCCAGTCTCAGTGTGTGGCCAAAGGCCTGATGCTGCTGTCACTCCTCATGCTGGCCATCCTC
  CTCAGGCACCCCTGGGTCAGGATGTGCAAAGCCCGGGACTCAGCTGCCATCTACCATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC058375
• Insert Size: 1740 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC058375, AAH58375
• RefSeq Size: 2339 bp
• RefSeq ORF: 1739 bp
• Locus ID: 67978
• Cytogenetics: 5 F
• Gene Summary: Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Required for hedgehog signaling transduction.[UniProtKB/Swiss-Prot Function]