Iigp1 (NM_001146275) Mouse Untagged Clone

CAT#: MC217812

Iigp1 (untagged) - Mouse interferon inducible GTPase 1 (Iigp1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Iigp1
• Synonyms: 2900074L10Rik; AI046432; AW111922; Ifgga1; Iigp; Irga6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217812 representing NM_001146275
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCAGCTGTTCTCTTCACCTAAGAGTGATGAGAATAATGATTTGCCCTCCAGCTTTACTGGTTATT
  TTAAGAAATTTAATACGGGAAGAAAAATCATTTCTCAAGAGATCCTCAATTTGATTGAATTAAGGATGAG
  AAAAGGGAATATTCAGTTGACAAACTCTGCAATCAGTGATGCATTAAAAGAAATCGATAGTAGTGTGCTC
  AATGTTGCTGTCACCGGGGAGACGGGATCAGGGAAGTCCAGCTTCATCAATACCCTGAGAGGCATTGGGA
  ATGAAGAAGAAGGTGCAGCTAAAACTGGGGTGGTGGAGGTAACCATGGAAAGACATCCATACAAACACCC
  CAATATACCCAATGTGGTTTTTTGGGACCTGCCTGGGATTGGAAGCACAAATTTCCCACCAAACACTTAC
  CTGGAGAAAATGAAGTTCTATGAGTACGATTTCTTCATTATTATTTCGGCCACACGCTTCAAGAAAAATG
  ATATAGACATTGCCAAAGCAATCAGCATGATGAAGAAGGAATTCTACTTCGTGAGAACCAAGGTGGACTC
  TGACATAACAAATGAAGCAGATGGCAAACCTCAAACCTTTGACAAAGAAAAGGTCCTGCAGGACATCCGC
  CTTAACTGTGTGAACACCTTTAGGGAGAATGGCATTGCTGAGCCACCAATCTTCCTGCTCTCTAACAAAA
  ATGTTTGTCACTATGACTTCCCCGTCCTGATGGACAAGCTGATAAGTGACCTCCCTATCTACAAGAGACA
  CAATTTTATGGTCTCCTTACCCAATATCACAGATTCAGTCATTGAAAAGAAGCGGCAATTTCTGAAGCAG
  AGGATTTGGCTGGAAGGATTTGCTGCTGACCTAGTGAATATCATCCCTTCTCTGACCTTTCTCTTGGACA
  GTGATTTGGAGACTCTGAAGAAAAGCATGAAATTCTACCGCACTGTGTTTGGAGTGGATGAAACATCTTT
  GCAGAGATTAGCTAGGGACTGGGAAATAGAGGTGGATCAGGTGGAGGCCATGATAAAATCTCCTGCTGTG
  TTCAAACCTACAGATGAAGAAACAATACAAGAAAGGCTTTCAAGATATATTCAGGAGTTCTGTTTGGCTA
  ATGGGTACTTACTTCCTAAAAATAGTTTTCTTAAAGAAATATTTTACCTGAAATATTATTTCCTTGACAT
  GGTGACTGAGGATGCTAAAACTCTTCTTAAAGAGATATGTTTAAGAAACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001146275
• Insert Size: 1242 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146275.1, NP_001139747.1
• RefSeq Size: 3149 bp
• RefSeq ORF: 1242 bp
• Locus ID: 60440
• UniProt ID: Q9QZ85
• Cytogenetics: 18 D3
• Gene Summary: GTPase with low activity. Has higher affinity for GDP than for GTP. Plays a role in resistance to intracellular pathogens. Required for disruption of the parasitophorous vacuole formed following T.gondii infection and subsequent killing of the parasite. Mediates resistance to C.trachomatis infection by targeting bacterial inclusions to autophagosomes for subsequent lysosomal destruction.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.