D11Bwg0517e (BC054403) Mouse Untagged Clone

CAT#: MC217793

D11Bwg0517e (untagged) - Mouse DNA segment, Chr 11, Brigham & Women's Genetics 0517 expressed (cDNA clone MGC:62856 IMAGE:6494361), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: D11Bwg0517e
• Synonyms: Fox-3; Hrnbp3; NeuN; Neuna60
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC054403
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGCCCTACCCCCCTGCCCAGTACCCTCCTCCGCCTCAGAATGGAATCCCAGCTGAATATGCCC
  CACCTCCGCCTCATCCCACCCAGGACTACTCCGGCCAGACCCCTGTTCCCCCCGAGCACGGCATGACCCT
  CTACACACCCGCACAGACTCATCCTGAGCAGCCAGGCACTGAGGCCAGCACACAGCCCATTGCTGGGACC
  CAGACGGTGCCGCAGGCAGATGAAGCAGCACAGACAGACAACCAGCAACTCCACCCTTCCGACCCCACGG
  AGAAGCAGCAGCCCAAACGACTACATGTCTCCAACATCCCCTTCCGGTTCAGGGACCCCGACCTGCGGCA
  AATGTTCGGGCAATTCGGGAAAATTTTAGACGTGGAGATCATTTTTAACGAGCGGGGCTCCAAGGTCAAT
  AATGCCACAGCCCGGGTCATGACCAATAAGAAGCCTGGGAACCCATATGCCAATGGCTGGAAGCTAAACC
  CTGTGGTAGGAACAGTCTATGGGCCTGAATTCTATGCAGTGACCAGTTTCCCCTACCCCACCACGGGCAC
  CGCCGTTGCCTACCGGGGTGCACACCTGCGGGGCCGGGGCCGTGCTGTGTATAATACATTTCGAGCTGCA
  CCACCTCCACCCCCCATTCCAACTTATGGAGCGGCACTGGAGCAAACGCTTGTTAAAATGCCAGTCCCGT
  GGGCGGGGCTGGCACCGTGCCCCCTCCCTCCTCAGCAGACACCGGAGCCGGCCTACCCCACCTCTCCAGC
  GTTCCCACCACTCTCTTGTCCGTTTGCTTCCAGGGTCGTGTATCAGGATGGATTTTATGGTGCTGAGATT
  TATGGAGGCTATGCAGCTTACAGATATGCTCAGCCAGCAGCCGCCACCGCAGCAGCCTACAGTGACAGTT
  ATGGTAGAGTCTATGCCGCTGCTGATCCTTACCATCACACCATCGGCCCCACAGCAACCTACAGCATCGG
  AACCATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC054403
• Insert Size: 990 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC054403, AAH54403
• RefSeq Size: 1553 bp
• RefSeq ORF: 989 bp
• Locus ID: 52897
• Cytogenetics: 11 83.22 cM
• Gene Summary: Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).[UniProtKB/Swiss-Prot Function]