Klhl7 (NM_001161800) Mouse Untagged Clone

CAT#: MC217790

Klhl7 (untagged) - Mouse kelch-like 7 (Drosophila) (Klhl7), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Klhl7
• Synonyms: 2700038B03Rik; D5Ertd363e; SBBI26
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217790 representing NM_001161800
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCTCCGGCGTGGAAAAGAGTAGCAAGAAGAAAACTGAGAAGAAACTTGCGGCTCGCGAAGAAG
  CCAAGCTGCTGGCGGGGTTCATGGGCGTCATGAACAACATGAGGAAACAGAGAACACTGTGTGACGTGAT
  CCTCACTGTCCAGGAAAGAAAGATCCCCGCTCACCGCGTTGTCCTGGCTGCAGCCAGTCACTTCTTTAAC
  TTAATGTTCACAACTAACATGCTTGAATCTAAGTCCTTTGAAGTAGAACTCAAAGATGCTGAACCTGATA
  TTATTGAACAACTGGTGGAATTTGCTTATACTGCTAGAATTTCTGTGAATAGCAACAATGTTCAGTCTTT
  GTTAGATGCAGCAAATCAGTACCAGATTGAACCTGTGAAGAAAATGTGTGTTGATTTTTTGAAAGAACAA
  GTTGATGCTTCAAATTGTCTTGGTATAAGTGTGCTTGCAGAGTGTCTGGACTGTCCTGAACTGAAAGCTA
  CAGCTGATGACTTCATTCATCAGCATTTCACTGAGGTTTACAAAACAGATGAATTCCTGCAGCTTGATGT
  CAAGCGAGTGACACATCTTCTCAGCCAGGACACCCTGACTGTGAGAGCAGAGGACCAGGTTTATGACGCT
  GCCGTGAGGTGGTTGAAATATGATGAGCCTAACCGCCAGCCATTCATGGTTGATATCCTTGCTAAAGTCA
  GGTTTCCTCTTATATCGAAGAATTTCCTAAGTAAAACAGTACAAGCTGAACCACTTATTCAAGATAATCC
  TGAATGCCTTAAAATGGTGATAAGTGGAATGAGGTACCATCTGCTGTCTCCTGAGGATCGAGAAGAACTT
  GCAGGTGGCACACGGCCTAGAAGAAAGAAACATGATTACCGCATAGCTCTGTTTGGAGGCTCCCAACCAC
  AGTCCTGTAGATATTTTAACCCAAAGGATTACAGTTGGACAGACATTCGCTGCCCCTTTGAAAAACGAAG
  AGATGCAGCATGTGTGTTTTGGGACAATGTAGTATACATTTTGGGAGGTTCTCAGCTTTTCCCCATAAAG
  CGAATGGATTGCTACAATGTTGTAAAGGATAGCTGGTATTCCAAACTGGGCCCTCCAACACCTCGAGACA
  GCCTTGCTGCCTGTGCAGCAGAAGGCAAAATTTATACATCTGGAGGTTCAGAAGTAGGAAACTCGGCCCT
  GTATTTGTTTGAATGCTATGATACAAGGACCGAGAGTTGGCACACGAAGCCCAGCATGCTGACTCAGCGC
  TGCAGCCATGGGATGGTGGAGGCCAATGGCCTCATCTATGTTTGTGGTGGAAGCTTAGGAAACAATGTTT
  CTGGGAGAGTGCTTAGTTCCTGTGAAGTGTATGATCCTGCCACAGAAACGTGGTCTGGACAATGTGGAAT
  ATTACGATATCAAGTTAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001161800
• Insert Size: 1422 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161800.1, NP_001155272.1
• RefSeq Size: 3329 bp
• RefSeq ORF: 1422 bp
• Locus ID: 52323
• Cytogenetics: 5 10.67 cM
• Gene Summary: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex. The BCR(KLHL7) complex acts by mediating ubiquitination and subsequent degradation of substrate proteins. Probably mediates 'Lys-48'-linked ubiquitination (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.