Fgd2 (BC021845) Mouse Untagged Clone

CAT#: MC217772

Fgd2 (untagged) - Mouse FYVE, RhoGEF and PH domain containing 2 (cDNA clone MGC:30923 IMAGE:4016697), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fgd2
• Synonyms: Tcd-2; Tcd2; tcs-2; tcs2; ZFYVE4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC021845
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACAGCGAGTACGTGAAGAACTTTGAGCGGGCCGCGGAACTGCTGGCCACGTGGATGGACAAGTCTC
  AGCCCTTCCAGGAGGTGGTCACCCGCATCCAGTGCAGCGAGGCCTCCAGCAGCCTGACCCTGCAGCACCA
  CATGTTGGAGCCTGTGCAAAGAATCCCGCGGTACGAACTGCTGCTCAAGGAATATGTGCAGAAGCTGCCA
  GCCCAGGCCCCAGACCTCGAAGATGCCCAGAGAGCACTGGACATGATCTTCTCAGCTGCACAGCACTCCA
  ATGCAGCCATTGCAGAGATGGAGCGGCTGCAGGGCCTGTGGGATGTGTACCAGCGCCTGGGCTTGGAGGA
  TGACATCGTGGACCCCTCCAACACCCTGCTCCGAGAGGGCCCTGTTCTCAAGATCTCTTTCCGCCGCAGC
  GACCCAATGGAACGCTACCTGGTTTTGTTCAACAACATGCTTCTGTATTGTGTACCCCGAGTCCTCCAAG
  TGGGTGCCCAGTTCCAGGTGCGGACTCGCATCGATGTGGCCGGCATGAAGGTGCGGGAGCTGACCGATGC
  TGAGTTCCCACACTCCTTCCTGGTGTCCGGAAAGCAGCGCACACTGGAGCTGCAGGCCCGGTCCCGAGAT
  GAAATGGTTTCCTGGATGCAGGCCTGCCAGGCAGCCATTGACCAGGTTGAGAAGCGGAGTGAGACCTTCA
  AGGCTGCTGTCCAGGGACCTCAGGGGGACACGCAGGAGCCCAAGCCACAGGTAGAGGAGCTGGGTCTCCG
  AGCGCCTCAGTGGGTCCGGGACAAGATGGTGACCATGTGCATGCGCTGCCAGGAGCCCTTCAATGCCCTG
  ACTCGTCGGCGCCACCACTGCCGGGCTTGCGGCTATGTAGTGTGTGCCAAGTGCTCTGACTACCGTGCGG
  AGCTGAAATATGACAGCAACAGGCCCAACCGAGTCTGCCTGACCTGCTACACATTTCTCACTGGAAACGT
  ACTCCCTCAAGGCAAGGAGGACAAGAGGCGGGGCATCTTGGAGAAAGAGGCCTCAGCAGCACCCGAGCAG
  AGTCTGGTGTGCAGCTTCCTGCAGCTCATAGGAGACAAGTGCAGCAGGAGCCTTCCCCGGAGTTGGTGTG
  TGATCCCCCGGGATGACCCCCTTGTCCTGTATGTCTATGCAGCCCCCCAGGACACAAAGGCTCACACCTC
  CATCCCCCTACTGGGCTATCAGGTGATTTCAGGGCCCCAGGGGGACCCTCGGGTTTTCCAGCTGCAACAG
  TCAGGCCAGCAGTACACCTTCAAGGCCGAGTCTGTGGAGCTGCAGGGCCGCTGGGTGACAGCTATCAAGC
  GTGCGGCCAGTGGCCGGACCCCTGAGGGACCTGACGAAGAAGATGTGTCTGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC021845
• Insert Size: 1386 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC021845, AAH21845
• RefSeq Size: 2094 bp
• RefSeq ORF: 1385 bp
• Locus ID: 26382
• Cytogenetics: 17 A3.3
• Gene Summary: Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Activates JNK1 via CDC42 but not RAC1. Binds to phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 5-monophosphate, phosphatidylinositol 4-monophosphate and phosphatidylinositol 3-monophosphate.[UniProtKB/Swiss-Prot Function]